Pairwise Alignments
Query, 1538 a.a., ATP-dependent helicase from Escherichia coli ECRC99
Subject, 1434 a.a., putative ATP-dependent DNA helicase lhr from Pseudomonas putida KT2440
Score = 598 bits (1542), Expect = e-174
Identities = 475/1505 (31%), Positives = 705/1505 (46%), Gaps = 188/1505 (12%)
Query: 5 PNPSSPLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARGEHALVIAPTGSGKTLAAFLY 64
P P+ ++F PA WF R F T Q+Q W + G+ L+ APTGSGKTL+AFL
Sbjct: 4 PAKHHPVLELFHPAVSTWFRRHFATVTDAQAQAWPLIHAGQSMLLAAPTGSGKTLSAFLA 63
Query: 65 ALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQRNLQIPLKGIADERRRRGETEV 124
LD L REG + E + + ++Y+SP+KAL D++ NLQ PL+GI+ +G
Sbjct: 64 VLDELLREGMKHQGELPAQ--TLVIYVSPLKALSNDIRLNLQAPLEGISQAIAEQGLNAP 121
Query: 125 NLRVGIRTGDTPAQERSKLTRNPPDILITTPESLYLMLTSRA-RETLRGVETVIIDEVHA 183
+ +RTGDTP +ER+ + + P IL+TTPESLY+++ S + RE L V TVI+DE+HA
Sbjct: 122 RITTAVRTGDTPQKERAAMRKLAPHILVTTPESLYVLMGSASGREGLAHVHTVIVDEIHA 181
Query: 184 VAGSKRGAHLALSLERLDALLHTSAQRIGLSATVRSASDVAAFL-GGDRPVTVVNPPAMR 242
+AG+KRGAHLAL+LERL AL +RIGLSAT R VA FL G RP +V+ R
Sbjct: 182 LAGNKRGAHLALTLERLQALCKKPLRRIGLSATQRPVERVAQFLVGSGRPCAIVDIGHAR 241
Query: 243 HPQIRIVVPVANMDDVSSVASGTGEDSHAGREGSIWPYIETGILDEVLRHRSTIVFTNSR 302
+ I VP + V ++ +W + + HR+T+VF N+R
Sbjct: 242 KRDLAIEVPPVPLGAVMAI--------------DVWGLVYDRLATLAREHRTTLVFVNTR 287
Query: 303 GLAEKLTARLNELYAARLQRSPSIAADAAHFESTSGATSNRVQSSDVFIARSHHGSVSKE 362
LAE++T L++ RL + ++AA HHGS+SK
Sbjct: 288 RLAERITRHLSD----RLGKE-AVAA--------------------------HHGSLSKA 316
Query: 363 QRAITEQALKSGELRCVVATSSLELGIDMGAVELVIQVATPLSVASGLQRIGRAGHQIGG 422
R EQ LKSG+L+ +VAT+SLELGID+G ++LV QVA+P S+A+ LQR+GRAGHQ+ G
Sbjct: 317 LRLDAEQRLKSGQLQVLVATASLELGIDIGDIDLVCQVASPGSIAAFLQRVGRAGHQVDG 376
Query: 423 ISKGLFFPRTRRDLVDSAVIVECMFAGRLENLTPPHNPLDVLAQHTVAAAAMDALQVDEW 482
+ KG FP +R DL++ +++C+ G L+ L P PLDVLAQ VA A+ A E
Sbjct: 377 VPKGRLFPTSRDDLIECVALLDCVRQGELDALHIPRAPLDVLAQQIVAEASNQAWHEQEL 436
Query: 483 YSRVRRAAPWKDLPRRVFDATLDMLSGRYPSGDFSAFRPKLVWNRETGILTARPGAQLLA 542
+R+A P+ +L + + A L ML+ Y +G L + +G L R G+QL A
Sbjct: 437 LDCLRQATPYAELDQHHYQALLRMLAEGY-NGRQGVRSAYLHRDAVSGTLRGRRGSQLTA 495
Query: 543 VTSGGTIPDRGMYSVLLPEGEEQAGSRRVGELDEEMVYESRVNDIITLGATSWRIQQITR 602
+TSGGTIPD Y+VLL E QA +G ++E+ ES DI LG S+RI ++
Sbjct: 496 LTSGGTIPDNADYAVLL---EPQA--LNIGSVNEDFAVESIAGDIFQLGNASYRILRVEP 550
Query: 603 DQVIVTPAPGRSARLPFWRGEGNGRPAELGEMIGDFLHLLADGAFFSG----TIPPWL-- 656
+V V A G +PFW GE GR EL + + +G + WL
Sbjct: 551 GRVRVEDAHGLPPTIPFWLGEAPGRSDELSAAVARLQARIDQQLALAGGDTVEVLAWLQA 610
Query: 657 ---AEENTIANIQGLIDEQRNATGIVPGSRHLVLERCRDEIGDWRIILHSPYGRRVHEPW 713
+E + + R G +P LV+ER DE G ++I+HSPYG R++ W
Sbjct: 611 TFELDEACAGQLLDYLARTREVLGALPSQDTLVMERFFDESGGTQLIIHSPYGSRINRAW 670
Query: 714 ALAIAGRIHALWGADASVVASDDGIVARIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSS 773
LA+ R + + AS+D IV + + D I+ +A+ +
Sbjct: 671 GLALRKRFCRTFNFELQAAASEDAIVLSLSTSHSFELDEVWRYLNSRSAEPILIQALLDA 730
Query: 774 ALFAARFRECAARALLMPGRTPGHRTPLWQQRLRASQLL------EIA--------QGYP 819
LF R+R A A+ +P G + QR+++ L+ +IA + P
Sbjct: 731 PLFGVRWRWNAGVAMALPRFVGGRKVAPQIQRMKSEDLVAAVFPDQIACLENIAGERQIP 790
Query: 820 DFPAILETLRECLQDVYDLPALERLMRRLNGGEIQISDVTTTTPSPFATSLLFGYVAEFM 879
D P + +TL +CL + D L+RR+ G +++ PSP A+++L +
Sbjct: 791 DHPLVEQTLDDCLHEAMDSEGWLALLRRMESGAVRLLCRDLPAPSPLASAIL--NARPYA 848
Query: 880 YQSDAPLAERRASVL---------------SLDSELLRNLLGQVDPGELLDPQVIRQVEE 924
+ DAPL ERR + +LD++ + + + P P ++ E
Sbjct: 849 FLDDAPLEERRTQAVLNRRWNDVHSSDDLGALDADAIAAVAAEAWP----QPANPDEMHE 904
Query: 925 ELQRL-APGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEIASYLENLLTVKRIFPT 983
L L A G+ +DLL L + A R +A NLL ++PT
Sbjct: 905 ALMSLGAIGQVEVDANPCWDLL--LRQLAKAGRALRLPDDKLWLARERLNLLLA--LYPT 960
Query: 984 --MISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLRYLRAHALVTAE 1041
ERLA D + L +L L H T
Sbjct: 961 AHREPALERLAGFDQPI--------------------EPDHALSELLRARLSGHGPQTLA 1000
Query: 1042 QLAHEFSLGIAIVEEQLQQLREQGLVM---------NLQQDIWVSDEVFRRLRLRSLQAA 1092
Q+A + +E+ L +L +G V+ +LQ W + R+ +++
Sbjct: 1001 QIAAPLGKPVTDIEQALARLEAEGYVLRGHFSPGATDLQ---WCERHLLARIHRYTVKRL 1057
Query: 1093 REATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQLAGVGLP 1152
R PV+ + R L + Q + P G V V+ QL G
Sbjct: 1058 RREIEPVSLQDFMRFLFDWQHLAPDER-------------LRGPQAVAEVLGQLQGFPSA 1104
Query: 1153 ASLWESQILPARVHDYSPEVLDELLATGAVIWSGQKKLGEDDGLVALHL------QEYAA 1206
A+ WE+++LPAR+ DYSP LDE +G WS L + L Q
Sbjct: 1105 AAAWEAELLPARIKDYSPHWLDEACRSGQFAWSRLAATVASSTLTSTPLVLLPRDQLNTW 1164
Query: 1207 ESFTPAEADQANRSALQQAIVAVLADGGAWFAQQISQRIRDKIGESVDPSALQEALWALV 1266
S PA A A Q+ A+ G +F + + + PS L+ AL LV
Sbjct: 1165 RSLAPAPAVDALGLRAQRVHEALKTQGALFFDELAHE-------AHLLPSELETALQELV 1217
Query: 1267 WQGVITSDIWAPLRALTRSSSNARTSTRRSHRARRGRPVYAQPVSPRVSYNTPNLAGRWS 1326
G+ +D + LR+L ++ R S +RRG P P+S +++ AGRW+
Sbjct: 1218 GSGLARADSFTGLRSLITPATK-----RSSRNSRRGHP----PLSSSMAH-----AGRWA 1263
Query: 1327 LLQVE--PLNDTERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQTLCR---SMEDSG 1381
LL+ P + +R+ +A +L RYG+I + + E+ P + L R +E G
Sbjct: 1264 LLRRSDAPPDHDQRLEHIARTLLRRYGVICWRLLARES--DVLPPWRELLRCYHRLEARG 1321
Query: 1382 RIMRGRFVEGLGGAQFAERLTIDRLRDLATQAAQTRHYTPVALSANDPANVWGNLLPWPA 1441
I GRF+ GL G QFA + LR + + T +SA DP N+ G+LLP
Sbjct: 1322 EIRGGRFIAGLAGEQFALPEAVVLLRQVRRREPDG---TLQVVSAGDPLNLIGSLLPGAK 1378
Query: 1442 HPATL 1446
PA +
Sbjct: 1379 VPAVI 1383
Score = 29.3 bits (64), Expect = 0.003
Identities = 17/51 (33%), Positives = 23/51 (45%)
Query: 1410 ATQAAQTRHYTPVALSANDPANVWGNLLPWPAHPATLVPTRRAGALVVVSG 1460
AT A+ T TP+ L D N W +L P PA A + +R + G
Sbjct: 1141 ATVASSTLTSTPLVLLPRDQLNTWRSLAPAPAVDALGLRAQRVHEALKTQG 1191