Pairwise Alignments

Query, 486 a.a., tRNA-N(6)-(isopentenyl)adenosine-37 thiotransferase enzyme MiaB from Echinicola vietnamensis KMM 6221, DSM 17526

Subject, 474 a.a., tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB from Escherichia coli ECRC102

 Score =  291 bits (744), Expect = 4e-83
 Identities = 172/454 (37%), Positives = 261/454 (57%), Gaps = 17/454 (3%)

Query: 33  KKLYIESYGCQMNFSDSEIVASIMKK-NGFDTTSNFEQADVIFLNTCSIREKAELTVRKR 91
           KKL+I+++GCQMN  DS  +A ++   +G+  T   E+ADV+ LNTCSIREKA+  V  +
Sbjct: 3   KKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVLLLNTCSIREKAQEKVFHQ 62

Query: 92  LTQFNTIKKARPDLTIGVLGCMAERLKDKLLEEEKLVDVVVGPDAYRDLPNLVKVAEEGD 151
           L ++  +K+  PDL IGV GC+A +  + + +    VD++ GP     LP ++  +  GD
Sbjct: 63  LGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDIIFGPQTLHRLPEMIN-SVRGD 121

Query: 152 KGVNTFLSREETYADISPVRLNTNGVSAFISIMRGCDNMCSFCVVPFTRGRERSRDPHSI 211
           +     +S  E             G +AF+SIM GC+  C++CVVP+TRG E SR    I
Sbjct: 122 RSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKYCTYCVVPYTRGEEVSRPSDDI 181

Query: 212 VKEAQELFDKGYKEVTLLGQNVDSYKWSPEENNKARLNKQEEDVSEVINFANLLEMVAKV 271
           + E  +L  +G +EV LLGQNV++++    +                 +FA+LL +VA +
Sbjct: 182 LFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTG-------------SFADLLRLVAAI 228

Query: 272 APQLRVRFSTSHPKDITDEVLYTMKKYDNICKYIHLPVQSGNSRILDLMNRTYDREWYLE 331
               R+RF+TSHP + TD+++   +    +  ++HLPVQSG+ RIL+LM RT+    Y  
Sbjct: 229 DGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNLMGRTHTALEYKA 288

Query: 332 RVAKIREILGQECGISSDMIAGFCSETEEEHQETLSLMDIVKYDFSYMFFYSERPGTLAA 391
            + K+R     +  ISSD I GF  ET E+ ++T+ L+  V +D SY F +S RPGT AA
Sbjct: 289 IIRKLR-AARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSYSFIFSARPGTPAA 347

Query: 392 KKYEDDIPLETKKRRLQEIIAKQTQHSLERNKLDLGQTQEVLVEGTSKRSEEQLKGRNSA 451
               DD+P E KK+RL  +  +  Q ++  ++  LG TQ +LVEGTS++S  +L GR   
Sbjct: 348 DMV-DDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGTSRKSIMELSGRTEN 406

Query: 452 NKVVIFPKGNHKKGDYVRVKIVDCTAATLFGEVL 485
           N+VV F       G +V V+I D    +L G+V+
Sbjct: 407 NRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVV 440