Pairwise Alignments
Query, 557 a.a., Putative fatty-acid--CoA ligase FadD21 from Azospirillum sp. SherDot2
Subject, 4326 a.a., Linear gramicidin synthase subunit D from Pseudomonas sp. RS175
Score = 192 bits (487), Expect = 3e-52
Identities = 157/551 (28%), Positives = 245/551 (44%), Gaps = 38/551 (6%)
Query: 22 PAEGMDRRLSELRTRSGVLAAGLAARGIRPGDRVVMLLDTGSAAICLLLALWQLQAVPVP 81
P + + +L R+ +AA L A GDR V+L +G + + VP
Sbjct: 35 PEQSVVLSYRDLDLRARTIAAALQAEAT-VGDRAVLLFPSGPDYVAAFFGCLYAGVIAVP 93
Query: 82 LRPWSGAVLETASERAGGFLRFLLEVTRRSDARLVVTD---RPAVVP-QLDPLLTTVEAL 137
P A ER LL + ++ RL++T R A+ + P L V++L
Sbjct: 94 AYPPESA-RRHHQER-------LLSIIADAEPRLLLTSSDLREALQAIESAPSLLCVDSL 145
Query: 138 EAPLERS--PPTGGDGECRADGLALIQFSSGSTGQPKGVMVTHAMAMAQLRQLADNYAAA 195
+ PT D D +A +Q++SGST PKGV V+H +A +L +
Sbjct: 146 DPARAGQWVAPTLQD-----DDIAFLQYTSGSTALPKGVQVSHGNLVAN--ELLIRHGFG 198
Query: 196 GSGRPPRSIASWLPFYHDMGLFIGILLPLFTGADAMVAPPAWYMRNPARWFAAMAARGSD 255
P I SWLP YHDMGL G+L P+F+G ++ P +++ P RW A++ G
Sbjct: 199 IDLNPDDVIVSWLPLYHDMGLIGGLLQPVFSGVPCVLMSPGYFLARPLRWLEAISEYGGT 258
Query: 256 LNFSTSSVLAATLRGVRRLHGRGCDLSAL-ILYVAAEKISPAVLDEAAAVLAPLGMPPSQ 314
++ + + DLS + Y +E I L+ A A G
Sbjct: 259 ISGGPDFAYRLCSERISDSALQRLDLSGWRVAYSGSEPIRLDTLERFAEKFAACGFTSDN 318
Query: 315 LRAGYGMAEYTLGCTHS-REPGLRRLRVRIAPGGRVLADGGNGCGGGGETLDLVSVGRPN 373
A YG+AE TL + R G+ LR+ A + A+ G G + G+P
Sbjct: 319 FMASYGLAEATLFVAGTPRSHGIASLRIDDAALAQNRAEPGQG-----SAVMSCGFGQPG 373
Query: 374 DACDLVVRDEAGCPLGEMRLGEITVAGPCLTPGYWRDEAMTQRAL----GRGWMRSGDLG 429
LVV PL + ++GE+ GP + GYWR+ T + GR W+R+GDLG
Sbjct: 374 HGV-LVVEPNTLQPLADQQVGEVWATGPSIAHGYWRNPEATAKTFVQHDGRTWLRTGDLG 432
Query: 430 FLHDGELFFVARKDEMLVVGGRNVIPADVELEVE-SLPFVGPGRAVLFAADEPGGPRQVL 488
F+H GELF R +ML+V G N+ P D+E +E + V GR FA ++ G +
Sbjct: 433 FVHGGELFITGRLKDMLIVRGHNLYPQDIEQTIEREVEVVRKGRVAAFAVND-GTEEGIG 491
Query: 489 LVEGNATSPPPVV--SGRLTAIRRCILDGFGFVPTDIAVVPKGTIEKTSSGKKRTAVIRR 546
+ + S ++ + +R+ + D P+ + ++ G + KTSSGK + + R
Sbjct: 492 IAAEISRSVQKILPPEALIKTLRQVVADACQQAPSVVVLLNPGALPKTSSGKLQRSACRT 551
Query: 547 RWQAGAIPQLA 557
R G++ A
Sbjct: 552 RLADGSLDSYA 562
Score = 52.4 bits (124), Expect = 4e-10
Identities = 114/482 (23%), Positives = 182/482 (37%), Gaps = 82/482 (17%)
Query: 24 EGMDRRLSELRTRSGVLAAGLAARGIRPGDRVVMLLDTGSAAICLLLALWQLQAVPVPLR 83
EG +EL T++ LA L +G+ P V + + + +LA+ + VPL
Sbjct: 1147 EGGRLDYAELHTQANRLAHYLRDKGVGPDVCVAIAAERSPQLLIGVLAIIKAGGAYVPLD 1206
Query: 84 PWSGAVLETASERAGGFLRFLLEVTRRSDARLVVTDRPAVVPQLDPLLTTVEALEA-PLE 142
+ +ER L ++L+ + D L T +P + + +V AL+A LE
Sbjct: 1207 A------DYPAER----LAYMLQDSG-VDLLLTQTALLERLPVCEGI--SVIALDALHLE 1253
Query: 143 RSPPTGGDGECRADGLALIQFSSGSTGQPKGVMVTHAMAMAQLRQLADNYAAAGSGRPPR 202
+ P D LA + ++SGSTGQPKGV THA +L+ + Y +
Sbjct: 1254 QWPGNPPGLHLHGDHLAYVIYTSGSTGQPKGVGNTHAALAERLQWMQATYGLDETD---- 1309
Query: 203 SIASWLPFYHDMGLFIGILLPLFTGADAMVAPPAWYMRNPARWFAAMAARGSD------- 255
+ P D+ ++ PL TG ++A P + R+P R + G
Sbjct: 1310 VLMQKAPISFDVSVW-ECFWPLITGCPLVLAGPGEH-RDPHRIAQLVQQFGVTTLHFVPP 1367
Query: 256 -LNFSTSSVLAATLRGVRRLHGRGCDLSALILYVAAEKISPAVLDEAAAVLAPLGMPPSQ 314
L+ L+A +RR+ G L A + + VLA L P +Q
Sbjct: 1368 LLSLFVDEPLSAGCTCLRRVFSGGEALPA---------------ELRSRVLAQL--PTAQ 1410
Query: 315 LRAGYGMAEYTLGCTHSREPGLRRLRVRIAPGGRVLADGGNGCGGGGETLDLVSVGRPND 374
L YG E + TH ADG + +GRP
Sbjct: 1411 LHNRYGPTETAINVTHWHCSD---------------ADG-----------ERSPIGRPLG 1444
Query: 375 ACDLVVRDEAGCPLGEMRLGEITVAGPCLTPGYWRDEAMTQR--------ALGRGWMRSG 426
V D P+ GE+ + G L GY ++ A G R+G
Sbjct: 1445 NVLCRVLDSELNPVPSGVSGELCIGGLGLARGYLGRPGLSAERFVPDPLAATGARLYRTG 1504
Query: 427 DLG-FLHDGELFFVARKDEMLVVGGRNVIPADVELEVESLPFVGPGRAVLFAADEPGGPR 485
D + G + ++ R D+ + + G + P E+E L G +A + D GP+
Sbjct: 1505 DRARWTAGGVIEYLGRLDQQVKLRGFRIEPQ--EIEARLLAQEGIAQAAVLVRDTAAGPQ 1562
Query: 486 QV 487
+
Sbjct: 1563 LI 1564
Score = 40.8 bits (94), Expect = 1e-06
Identities = 112/488 (22%), Positives = 166/488 (34%), Gaps = 98/488 (20%)
Query: 25 GMDRRLSELRTRSGVLAAGLAARGIRPGDRVVMLLDTGSAAICLLLALWQLQAVPVPLRP 84
G EL R+ LA L RG+ P RV + L+ + LLA+ + VPL P
Sbjct: 2206 GQTLTYGELERRANRLAWMLRERGVGPQVRVGLALERSLEMVIGLLAILKAGGAYVPLDP 2265
Query: 85 WSGAVLETASERAGGFLRFLLEVTRRSDARLVVTDRPAVVPQLDPLLTTVEALEAPLERS 144
E +R L +++E S +++DR A+ L
Sbjct: 2266 ------EYPLDR----LHYMIE---DSGIAWLLSDR---------------AMFESLGAL 2297
Query: 145 PPTGGDGECRADGLALIQF-------------------SSGSTGQPKGVMVTHAMAMAQL 185
P G DG AL+Q+ +SGSTG+PKGV+V+H
Sbjct: 2298 PDGVGRWSLEEDGPALVQYPDHPLPSINLPQHQAYLIYTSGSTGKPKGVVVSHGEIAMHC 2357
Query: 186 RQLADNYAAAGSGRPPRSIASWLPFYHDMGLFIGILLPLFTGADAMV-APPAWYMRNPAR 244
+ + + RP + D +L+PL +GA ++ A W
Sbjct: 2358 QAVIQRFGM----RPDDCELHFYSINFDAAT-ERLLVPLLSGAHVVLRAQGQWDAEEICG 2412
Query: 245 WFAAMAARGSDLNFSTSSVLAATLRGVRRLHGRGCDLSALILYVAAEKISPAVLDEAAAV 304
A S S LA + L +G L + E ++ L A
Sbjct: 2413 LIRAHGVNVLGFTPSYGSQLA------QWLATQGQILPVRMCITGGEALTGEHLQRIRAA 2466
Query: 305 LAPLGMPPSQLRAGYGMAEYTLGCTHSREPGLRRLRVRIAPGGRVLADGGNGCGGGGETL 364
+ P++ YG E + P V P G V+ GE +
Sbjct: 2467 FS-----PARFFNAYGPTETVVMPLACLAPEQLEEGVTSVPIGSVV----------GERV 2511
Query: 365 DLVSVGRPNDACDLVVRDEAGCPLGEMRLGEITVAGPCLTPGYWRDEAMTQRAL------ 418
+ + D A P G GE+ V G L GY + MT
Sbjct: 2512 AYI-----------LDADLALVPQG--ATGELYVGGAGLAQGYHQRPGMTAERFVADPFC 2558
Query: 419 --GRGWMRSGDLGFLH-DGELFFVARKDEMLVVGGRNVIPADVELEVESLPFVGPGRAVL 475
G R+GDL DG + ++ R D + + G + E+E L AV+
Sbjct: 2559 TEGGRLYRTGDLVRQRADGLVEYLGRVDHQVKIRGFRIELG--EIETRLLEHDAVREAVV 2616
Query: 476 FAADEPGG 483
A D PGG
Sbjct: 2617 LALDMPGG 2624
Score = 40.8 bits (94), Expect = 1e-06
Identities = 44/150 (29%), Positives = 66/150 (44%), Gaps = 17/150 (11%)
Query: 33 LRTRSGVLAAGLAARGIRPGDRVVMLLDTGSAAICLLLALWQLQAVPVPLRPWSGAVLET 92
L S L L A G+ V +L + G + +++ ++ A +PL P
Sbjct: 3743 LNAHSNRLGHALIACGVGLDQPVALLAERGLDLLAMIIGSFKAGAGYLPLDPG------L 3796
Query: 93 ASERAGGFLRF----LLEVTRRSDAR-LVVTDRPAVVPQLDPLL-TTVEALEAPLERSPP 146
S+R G L LL T+ A+ L + D A + L+ ++A +A E
Sbjct: 3797 PSQRLGRILELSRTPLLVCTQACHAQALALLDEHACSARPKLLVWEDIQASDASQENPGV 3856
Query: 147 TGGDGECRADGLALIQFSSGSTGQPKGVMV 176
G D LA + ++SGSTGQPKGVMV
Sbjct: 3857 HSGP-----DNLAYVIYTSGSTGQPKGVMV 3881