SEED Subsystem: Thiamin biosynthesis in Pseudomonas putida KT2440

Experiments:

Role Gene Name  
1-deoxy-D-xylulose 5-phosphate synthase (EC 2.2.1.7) PP_0527
5-FCL-like protein No genes
ATPase component YkoD of energizing module of thiamin-regulated ECF transporter for HydroxyMethylPyrimidine No genes
Additional substrate-binding component of thiamin-regulated ECF transporter for HydroxyMethylPyrimidine No genes
Cysteine desulfurase (EC 2.8.1.7), IscS subfamily PP_0842
Glycine oxidase ThiO (EC 1.4.3.19) PP_0612
Hydroxyethylthiazole kinase (EC 2.7.1.50) No genes
Hydroxymethylpyrimidine ABC transporter, ATPase component No genes
Hydroxymethylpyrimidine ABC transporter, substrate-binding component PP_3213
Hydroxymethylpyrimidine ABC transporter, transmembrane component PP_3210
Hydroxymethylpyrimidine synthesis protein THI5 No genes
Phosphomethylpyrimidine kinase (EC 2.7.4.7) No genes
Predicted hydroxymethylpyrimidine transporter CytX PP_4921
Predicted thiamin transporter PnuT No genes
Predicted thiamin transporter ThiT No genes
Predicted thiazole transporter ThiU No genes
Putative cysteine desulfurase, associated with tRNA 4-thiouridine synthase No genes
Rhodanese-like domain required for thiamine synthesis No genes
Substrate-specific component ThiT of thiamin ECF transporter No genes
Substrate-specific component ThiW of predicted thiazole ECF transporter No genes
Substrate-specific component YkoE of thiamin-regulated ECF transporter for HydroxyMethylPyrimidine No genes
Sulfur carrier protein ThiS PP_5105
Sulfur carrier protein adenylyltransferase ThiF No genes
Thiamin ABC transporter, ATPase component No genes
Thiamin ABC transporter, substrate-binding component No genes
Thiamin ABC transporter, transmembrane component PP_1723
Thiamin biosynthesis protein ThiC No genes
Thiamin pyrophosphokinase (EC 2.7.6.2) No genes
Thiamin transport gene THI10 No genes
Thiamin-phosphate phosphatase (EC 3.1.3.-) No genes
Thiamin-phosphate pyrophosphorylase (EC 2.5.1.3) PP_4783
Thiamin-phosphate synthase ThiN (EC 2.5.1.3) No genes
Thiamin-regulated hypothetical deacetylase No genes
Thiamin-regulated outer membrane receptor Omr1 No genes
Thiaminase II (EC 3.5.99.2) PP_3185
  PP_3186
Thiamine kinase (EC 2.7.1.89) No genes
Thiamine-monophosphate kinase (EC 2.7.4.16) PP_0519
Thiazole biosynthesis protein ThiG PP_5104
Thiazole biosynthesis protein ThiH No genes
Thiazole biosynthetic enzyme Thi4 No genes
Transmembrane component YkoC of energizing module of thiamin-regulated ECF transporter for HydroxyMethylPyrimidine No genes
YjbQ (alternate ThiE) No genes
tRNA S(4)U 4-thiouridine synthase (former ThiI) No genes

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