SEED Subsystem: Serine-glyoxylate cycle in Pseudomonas putida KT2440

Experiments:

Role Gene Name  
3-ketoacyl-CoA thiolase (EC 2.3.1.16) PP_3355
5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20) PP_4638
  PP_4977
5-FCL-like protein, but predicted not to be 5-formyltetrahydrofolate cyclo-ligase (5-FCL) No genes
5-formyltetrahydrofolate cyclo-ligase (EC 6.3.3.2) PP_5203
Acetoacetyl-CoA reductase (EC 1.1.1.36) No genes
Acetyl-CoA acetyltransferase (EC 2.3.1.9) PP_4636
Aconitate hydratase (EC 4.2.1.3) PP_2112
Citrate synthase (EC 2.3.3.1) No genes
Citrate synthase (si) (EC 2.3.3.1) PP_4194
Enolase (EC 4.2.1.11) PP_1612
Formate--tetrahydrofolate ligase (EC 6.3.4.3) No genes
Formiminotetrahydrofolate cyclodeaminase (EC 4.3.1.4) No genes
Fumarate hydratase class I, aerobic (EC 4.2.1.2) PP_0897
Fumarate hydratase class I, anaerobic (EC 4.2.1.2) No genes
GDSL-like lipase/acylhydrolase No genes
Glycerate kinase (EC 2.7.1.31) PP_1212
  PP_2381
  PP_3134
  PP_3178
  PP_3962
Isocitrate lyase (EC 4.1.3.1) PP_4116
Malate dehydrogenase (EC 1.1.1.37) PP_0654
  PP_2835
Malate synthase-related protein No genes
Malyl-CoA lyase (EC 4.1.3.24) No genes
Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9) PP_1945
  PP_2265
Methylcrotonyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.4) PP_4067
Methylcrotonyl-CoA carboxylase carboxyl transferase subunit (EC 6.4.1.4) PP_4065
Methylene tetrahydromethanopterin dehydrogenase (EC 1.5.99.9) No genes
Methylenetetrahydrofolate dehydrogenase (NADP+) (EC 1.5.1.5) PP_1945
  PP_2265
Methylmalonyl-CoA mutase (EC 5.4.99.2) No genes
N(5),N(10)-methenyltetrahydromethanopterin cyclohydrolase (EC 3.5.4.27) No genes
Phosphoenolpyruvate carboxykinase [ATP] (EC 4.1.1.49) No genes
Phosphoglyceromutase No genes
Propionyl-CoA carboxylase beta chain (EC 6.4.1.3) No genes
Propionyl-CoA carboxylase carboxyl transferase subunit (EC 6.4.1.3) No genes
Putative malate dehydrogenase (EC 1.1.1.37), similar to archaeal MJ1425 No genes
Serine hydroxymethyltransferase (EC 2.1.2.1) PP_0322
  PP_0671
  PP_0672
Serine--glyoxylate aminotransferase (EC 2.6.1.45) No genes
Serine--pyruvate aminotransferase (EC 2.6.1.51) No genes
Serine-pyruvate aminotransferase/archaeal aspartate aminotransferase No genes
Succinate dehydrogenase flavoprotein subunit (EC 1.3.99.1) PP_4191
Succinate dehydrogenase iron-sulfur protein (EC 1.3.99.1) PP_4190
Succinyl-CoA ligase [ADP-forming] alpha chain (EC 6.2.1.5) PP_4185
Succinyl-CoA ligase [ADP-forming] beta chain (EC 6.2.1.5) PP_4186
Succinyl-CoA:3-ketoacid-coenzyme A transferase subunit A (EC 2.8.3.5) PP_3122
Succinyl-CoA:3-ketoacid-coenzyme A transferase subunit B (EC 2.8.3.5) PP_3123
crotonyl-CoA reductase No genes
cytosolic long-chain acyl-CoA thioester hydrolase family protein PP_1466
  PP_4975
  PP_5331
  PP_5356
decarboxylase No genes
isobutyryl-CoA dehydrogenase No genes
low-specificity D-threonine aldolase No genes
polyhydroxyalkanoate synthesis repressor PhaR No genes
putative glycerate kinase No genes
putative isobutyryl-CoA mutase, chain B No genes

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