SEED Subsystem: Siderophore Pyoverdine in Pseudomonas putida KT2440

Experiments:

Role Gene Name 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.  
ABC transporter in pyoverdin gene cluster, ATP-binding component PP_3802 +0.0
ABC transporter in pyoverdin gene cluster, periplasmic component PP_3804 -0.2
ABC transporter in pyoverdin gene cluster, permease component PP_3803 -0.5
Acyl-homoserine lactone acylase PvdQ (EC 3.5.1.-), quorum-quenching PP_2901 +0.2
Antisigma transmembrane sensor FpvR No genes
Cation ABC transporter, periplasmic cation-binding protein, PA2407 homolog No genes
Hypothetical MbtH-like protein, PA2412 homolog No genes
Hypothetical protein PvdX No genes
Hypothetical protein PvdY PP_4245 -0.0
Hypothetical protein in pyoverdin gene cluster No genes
Hypothetical protein in pyoverdin gene cluster, PA2405 homolog No genes
Hypothetical protein in pyoverdin gene cluster, PA2406 homolog No genes
L-ornithine 5-monooxygenase (EC 1.13.12.-), PvdA of pyoverdin biosynthesis PP_3796 +0.5
Non-ribosomal peptide synthetase modules, pyoverdine PP_3788 -0.0
Non-ribosomal peptide synthetase modules, pyoverdine?? No genes
Outer membrane ferripyoverdine receptor PP_0669 +0.0
Outer membrane ferripyoverdine receptor FpvA, TonB-dependent No genes
Outer membrane ferripyoverdine receptor FpvB, for Type I pyoverdine No genes
Outer membrane porin, coexpressed with pyoverdine biosynthesis regulon PP_0799 +0.2
Outer membrane pyoverdine eflux protein PP_4211 +0.1
Pirin-related protein, coexpressed with pyoverdine biosynthesis regulon No genes
Probable hydrolase, coexpressed with pyoverdine biosynthesis regulon No genes
Probable thioesterase involved in non-ribosomal peptide biosynthesis, PA2411 homolog No genes
Putative dipeptidase, pyoverdin biosynthesis PvdM PP_4213 -0.1
Putative iron-regulated membrane protein, PA2403 homolog No genes
Putative thiamine pyrophosphate-requiring enzyme, PA2404 homolog No genes
PvdE, pyoverdine ABC export system, fused ATPase and permease components PP_4216 N.D.
PvdO, pyoverdine responsive serine/threonine kinase (predicted by OlgaV) PP_4215 -0.2
Pyoverdin biosynthesis protein PvdH, L-2,4-diaminobutyrate:2-oxoglutarate aminotransferase (EC 2.6.1.76) PP_4223 -0.3
Pyoverdin biosynthesis protein PvdN, putative aminotransferase, class V PP_4214 -1.0
Pyoverdine biosynthesis related protein PvdP PP_4212 -0.3
Pyoverdine chromophore precursor synthetase PvdL No genes
Pyoverdine efflux carrier and ATP binding protein PP_4210 +0.2
Pyoverdine sidechain non-ribosomal peptide synthetase PvdD No genes
Pyoverdine sidechain non-ribosomal peptide synthetase PvdI No genes
Pyoverdine sidechain non-ribosomal peptide synthetase PvdJ No genes
Pyoverdine synthetase PvdF, N5-hydroxyornithine formyltransferase No genes
Sigma factor PvdS, controling pyoverdin biosynthesis PP_4244 +1.0
Sigma-70 factor FpvI (ECF subfamily), controling pyoverdin biosynthesis No genes
Thioesterase PvdG involved in non-ribosomal peptide biosynthesis No genes
pyoverdine-specific efflux macA-like protein PP_4209 +0.1

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