SEED Subsystem: Cysteine Biosynthesis in Escherichia coli BW25113

Experiments:

Role Gene Name LB Aerobic with Novobiocin 0.0000625 mM  
Adenylylsulfate kinase (EC 2.7.1.25) b2750 cysC +0.2
Cys regulon transcriptional activator CysB b1275 cysB N.D.
Cystathionine beta-synthase (EC 4.2.1.22) No genes
Cystathionine gamma-lyase (EC 4.4.1.1) No genes
Cysteine synthase (EC 2.5.1.47) b2414 cysK +0.6
Cysteine synthase A (EC 2.5.1.47) No genes
Cysteine synthase B (EC 2.5.1.47) b2421 cysM +1.6
Phosphoadenylyl-sulfate reductase [thioredoxin] (EC 1.8.4.8) b2762 cysH -0.0
Serine acetyltransferase (EC 2.3.1.30) b3607 cysE N.D.
Sulfate adenylyltransferase subunit 1 (EC 2.7.7.4) b2751 cysN -0.3
Sulfate adenylyltransferase subunit 2 (EC 2.7.7.4) b2752 cysD +0.3
Sulfate and thiosulfate binding protein CysP b2425 cysP -0.0
Sulfate and thiosulfate import ATP-binding protein CysA (EC 3.6.3.25) b2422 cysA +0.6
Sulfate permease No genes
Sulfate permease, Trk-type No genes
Sulfate transport system permease protein CysT b2424 cysU -0.1
Sulfate transport system permease protein CysW b2423 cysW +0.5
Sulfate transporter, CysZ-type b2413 cysZ -2.4
Sulfite reductase [NADPH] flavoprotein alpha-component (EC 1.8.1.2) b2764 cysJ +0.2
Sulfite reductase [NADPH] hemoprotein beta-component (EC 1.8.1.2) b2763 cysI +0.0

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