SEED Subsystem: Serine-glyoxylate cycle in Pseudomonas simiae WCS417

Experiments:

Role Gene Name 2-Deoxy-D-Ribose (C)  
3-ketoacyl-CoA thiolase (EC 2.3.1.16) PS417_13855 +0.0
  PS417_19210 -0.0
5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20) PS417_26510 -0.3
5-FCL-like protein, but predicted not to be 5-formyltetrahydrofolate cyclo-ligase (5-FCL) No genes
5-formyltetrahydrofolate cyclo-ligase (EC 6.3.3.2) PS417_27305 -0.8
Acetoacetyl-CoA reductase (EC 1.1.1.36) No genes
Acetyl-CoA acetyltransferase (EC 2.3.1.9) PS417_10515 -1.8
Aconitate hydratase (EC 4.2.1.3) PS417_07515 -0.2
Citrate synthase (EC 2.3.3.1) No genes
Citrate synthase (si) (EC 2.3.3.1) PS417_08870 N.D.
Enolase (EC 4.2.1.11) PS417_06310 N.D.
Formate--tetrahydrofolate ligase (EC 6.3.4.3) No genes
Formiminotetrahydrofolate cyclodeaminase (EC 4.3.1.4) No genes
Fumarate hydratase class I, aerobic (EC 4.2.1.2) PS417_22635 N.D.
Fumarate hydratase class I, anaerobic (EC 4.2.1.2) No genes
GDSL-like lipase/acylhydrolase No genes
Glycerate kinase (EC 2.7.1.31) PS417_13745 +0.1
  PS417_13970 -4.0
  PS417_22470 N.D.
Isocitrate lyase (EC 4.1.3.1) PS417_16570 -0.2
Malate dehydrogenase (EC 1.1.1.37) No genes
Malate synthase-related protein No genes
Malyl-CoA lyase (EC 4.1.3.24) No genes
Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9) PS417_17265 N.D.
Methylcrotonyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.4) PS417_17030 N.D.
  PS417_19595 +0.0
Methylcrotonyl-CoA carboxylase carboxyl transferase subunit (EC 6.4.1.4) PS417_17020 N.D.
  PS417_19580 -0.2
Methylene tetrahydromethanopterin dehydrogenase (EC 1.5.99.9) No genes
Methylenetetrahydrofolate dehydrogenase (NADP+) (EC 1.5.1.5) PS417_17265 N.D.
Methylmalonyl-CoA mutase (EC 5.4.99.2) No genes
N(5),N(10)-methenyltetrahydromethanopterin cyclohydrolase (EC 3.5.4.27) No genes
Phosphoenolpyruvate carboxykinase [ATP] (EC 4.1.1.49) PS417_01210 +0.1
Phosphoglyceromutase No genes
Propionyl-CoA carboxylase beta chain (EC 6.4.1.3) No genes
Propionyl-CoA carboxylase carboxyl transferase subunit (EC 6.4.1.3) No genes
Putative malate dehydrogenase (EC 1.1.1.37), similar to archaeal MJ1425 No genes
Serine hydroxymethyltransferase (EC 2.1.2.1) PS417_24275 -0.3
  PS417_24280 N.D.
  PS417_26195 -0.2
Serine--glyoxylate aminotransferase (EC 2.6.1.45) No genes
Serine--pyruvate aminotransferase (EC 2.6.1.51) No genes
Serine-pyruvate aminotransferase/archaeal aspartate aminotransferase No genes
Succinate dehydrogenase flavoprotein subunit (EC 1.3.99.1) PS417_08885 N.D.
Succinate dehydrogenase iron-sulfur protein (EC 1.3.99.1) PS417_08890 N.D.
Succinyl-CoA ligase [ADP-forming] alpha chain (EC 6.2.1.5) PS417_08915 N.D.
Succinyl-CoA ligase [ADP-forming] beta chain (EC 6.2.1.5) PS417_08910 N.D.
Succinyl-CoA:3-ketoacid-coenzyme A transferase subunit A (EC 2.8.3.5) PS417_10525 N.D.
Succinyl-CoA:3-ketoacid-coenzyme A transferase subunit B (EC 2.8.3.5) PS417_10520 -0.8
crotonyl-CoA reductase No genes
cytosolic long-chain acyl-CoA thioester hydrolase family protein PS417_22900 +0.3
  PS417_26500 +0.4
  PS417_28095 N.D.
  PS417_28260 +0.0
decarboxylase No genes
isobutyryl-CoA dehydrogenase No genes
low-specificity D-threonine aldolase PS417_12910 +0.3
polyhydroxyalkanoate synthesis repressor PhaR No genes
putative glycerate kinase No genes
putative isobutyryl-CoA mutase, chain B No genes

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