SEED Subsystem: TCA Cycle in Escherichia coli BW25113

Experiments:

Role Gene Name outer cut, LB soft agar motility assay  
⨯
2-oxoglutarate dehydrogenase E1 component (EC 1.2.4.2) b0726 sucA -1.1
Aconitate hydratase (EC 4.2.1.3) No genes
Aconitate hydratase 2 (EC 4.2.1.3) No genes
Aconitate hydratase X, predicted No genes
Aconitate hydratase large subunit (EC 4.2.1.3) No genes
Aconitate hydratase small subunit (EC 4.2.1.3) No genes
Archaeal succinyl-CoA ligase [ADP-forming] alpha chain (EC 6.2.1.5) No genes
Archaeal succinyl-CoA ligase [ADP-forming] beta chain (EC 6.2.1.5) No genes
Citrate lyase gamma chain, acyl carrier protein (EC 4.1.3.6) b0617 citD +0.5
Citrate synthase (si) (EC 2.3.3.1) b0720 gltA -1.7
Dihydrolipoamide dehydrogenase (EC 1.8.1.4) No genes
Dihydrolipoamide dehydrogenase of 2-oxoglutarate dehydrogenase (EC 1.8.1.4) No genes
Dihydrolipoamide dehydrogenase of pyruvate dehydrogenase complex (EC 1.8.1.4) No genes
Dihydrolipoamide succinyltransferase component (E2) of 2-oxoglutarate dehydrogenase complex (EC 2.3.1.61) b0727 sucB N.D.
Fumarate hydratase class I (EC 4.2.1.2) No genes
Fumarate hydratase class I, aerobic (EC 4.2.1.2) b1612 fumA -0.4
Fumarate hydratase class II (EC 4.2.1.2) b1611 fumC +0.2
Isocitrate dehydrogenase [NADP] (EC 1.1.1.42) b1136 icd N.D.
Isocitrate dehydrogenase [NAD] (EC 1.1.1.41) No genes
Malate dehydrogenase (EC 1.1.1.37) b0801 ybiC -0.1
  b3236 mdh -1.0
Malate:quinone oxidoreductase (EC 1.1.5.4) b2210 mqo -0.1
Putative Dihydrolipoamide dehydrogenase (EC 1.8.1.4) No genes
Putative malate dehydrogenase (EC 1.1.1.37), similar to archaeal MJ1425 No genes
Succinate dehydrogenase flavoprotein subunit (EC 1.3.99.1) b0723 sdhA -0.8
  b4154 frdA -0.9
Succinate dehydrogenase iron-sulfur protein (EC 1.3.99.1) b0724 sdhB -1.0
  b4153 frdB -1.9
Succinyl-CoA ligase [ADP-forming] alpha chain (EC 6.2.1.5) b0729 sucD -0.4
Succinyl-CoA ligase [ADP-forming] beta chain (EC 6.2.1.5) b0728 sucC -1.3
Transcriptional regulator of succinyl CoA synthetase operon No genes
hypothetical protein that often co-occurs with aconitase No genes

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