SEED Subsystem: Branched-Chain Amino Acid Biosynthesis in Escherichia coli BW25113

Experiments:

Role Gene Name M63 no sulfur + 5 mM L-cys  
(R)-citramalate synthase (EC 2.3.1.182) No genes
2-isopropylmalate synthase (EC 2.3.3.13) b0074 leuA -5.2
3-isopropylmalate dehydratase large subunit (EC 4.2.1.33) b0072 leuC -5.6
3-isopropylmalate dehydratase small subunit (EC 4.2.1.33) b0071 leuD -5.0
3-isopropylmalate dehydrogenase (EC 1.1.1.85) b0073 leuB -4.9
Acetolactate synthase large subunit (EC 2.2.1.6) b3671 ilvB -3.2
  b0077 ilvI -0.9
Acetolactate synthase small subunit (EC 2.2.1.6) b0078 ilvH -0.7
  b3670 ilvN -1.9
  b3769 ilvM +0.2
Acetolactate synthase small subunit (EC 2.2.1.6), Xanthomonadales type No genes
Acetolactate synthase small subunit (EC 2.2.1.6), predicted No genes
Acetolactate synthase small subunit (EC 2.2.1.6), predicted, Archaeal type No genes
Acetolactate synthase small subunit (EC 2.2.1.6), predicted, Archaeal type 2 No genes
Branched-chain amino acid aminotransferase (EC 2.6.1.42) b3770 ilvE -5.0
Dihydroxy-acid dehydratase (EC 4.2.1.9) b0269 yagF -0.0
  b4297 yjhG -0.0
  b3771 ilvD -4.8
Ketol-acid reductoisomerase (EC 1.1.1.86) b3774 ilvC -5.3
Leucine dehydrogenase (EC 1.4.1.9) No genes
Leucine-responsive regulatory protein, regulator for leucine (or lrp) regulon and high-affinity branched-chain amino acid transport system b0889 lrp -1.6
Threonine dehydratase (EC 4.3.1.19) No genes
Threonine dehydratase biosynthetic (EC 4.3.1.19) b3772 ilvA -5.3
Threonine dehydratase, catabolic (EC 4.3.1.19) b3117 tdcB -0.3
Valine--pyruvate aminotransferase (EC 2.6.1.66) b3572 avtA -0.4

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