SEED Subsystem: Acetyl-CoA fermentation to Butyrate in Pseudomonas putida KT2440

Experiments:

Role Gene Name Levulinic Acid (C)  
⨯
3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) PP_2047 -0.1
  PP_2136 -0.2
3-hydroxybutyrate dehydrogenase (EC 1.1.1.30) No genes
3-hydroxybutyryl-CoA dehydratase (EC 4.2.1.55) No genes
3-hydroxybutyryl-CoA dehydrogenase (EC 1.1.1.157) PP_3755 -2.4
3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3) PP_2047 -0.1
  PP_2136 -0.2
Acetoacetate metabolism regulatory protein AtoC No genes
Acetoacetyl-CoA reductase (EC 1.1.1.36) No genes
Acetyl-CoA acetyltransferase (EC 2.3.1.9) PP_4636 -0.2
Acetyl-CoA:acetoacetyl-CoA transferase, alpha subunit (EC 2.8.3.8) No genes
Acetyl-CoA:acetoacetyl-CoA transferase, beta subunit (EC 2.8.3.8) No genes
Butyrate kinase (EC 2.7.2.7) No genes
Butyrate-acetoacetate CoA-transferase subunit A (EC 2.8.3.9) No genes
Butyrate-acetoacetate CoA-transferase subunit B (EC 2.8.3.9) No genes
Butyryl-CoA dehydrogenase (EC 1.3.99.2) PP_1893 +0.0
  PP_2216 -0.2
  PP_2793 -5.3
  PP_3638 -0.1
  PP_3725 -0.1
Electron transfer flavoprotein, alpha subunit PP_0312 -0.3
  PP_4201 N.D.
Electron transfer flavoprotein, beta subunit PP_0313 -0.4
  PP_4202 N.D.
Electron transfer flavoprotein-ubiquinone oxidoreductase (EC 1.5.5.1) PP_4203 N.D.
Enoyl-CoA hydratase (EC 4.2.1.17) PP_1412 -0.1
  PP_2047 -0.1
  PP_2136 -0.2
  PP_3726 -0.3
  PP_3732 +0.0
Ferredoxin-like protein No genes
Ferredoxin-like protein FixX No genes
Ferredoxin-like protein YdiT No genes
Ferredoxin-like protein YgcO No genes
Probable electron transfer flavoprotein-quinone oxidoreductase FixC (EC 1.5.5.-) No genes
phosphotransbutyrylase (EC 2.3.1.19) No genes

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