SEED Subsystem: Polyhydroxybutyrate metabolism in Pseudomonas putida KT2440

Experiments:

Role Gene Name Levulinic Acid (C)  
⨯
3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) PP_2047 -0.0
  PP_2136 +0.1
3-hydroxybutyryl-CoA dehydratase (EC 4.2.1.55) No genes
3-hydroxybutyryl-CoA dehydrogenase (EC 1.1.1.157) PP_3755 -2.4
3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3) PP_2047 -0.0
  PP_2136 +0.1
3-ketoacyl-CoA thiolase (EC 2.3.1.16) PP_3355 +0.1
Acetoacetyl-CoA reductase (EC 1.1.1.36) No genes
Acetoacetyl-CoA synthetase (EC 6.2.1.16) PP_3071 -0.0
  PP_3458 -0.2
Acetyl-CoA acetyltransferase (EC 2.3.1.9) PP_4636 -0.2
Butyrate-acetoacetate CoA-transferase subunit A (EC 2.8.3.9) No genes
Butyrate-acetoacetate CoA-transferase subunit B (EC 2.8.3.9) No genes
D(-)-3-hydroxybutyrate oligomer hydrolase (EC 3.1.1.22) No genes
D-beta-hydroxybutyrate dehydrogenase (EC 1.1.1.30) PP_2784 +0.3
  PP_3073 -0.4
D-beta-hydroxybutyrate permease PP_3074 +0.2
Enoyl-CoA hydratase (EC 4.2.1.17) PP_1412 -0.0
  PP_2047 -0.0
  PP_2136 +0.1
  PP_3726 -0.5
  PP_3732 +0.3
Intracellular PHB depolymerase (EC 3.1.1.-) No genes
Poly(3-hydroxyalkanoate) depolymerase PP_5004 -0.1
Polyhydroxyalkanoate granule-associated protein PhaF PP_5007 -0.3
Polyhydroxyalkanoic acid synthase PP_5003 +0.2
  PP_5005 +0.2
Putative 3-hydroxyacyl-coa dehydrogenase No genes
Short chain fatty acids transporter PP_3124 -0.0
polyhydroxyalkanoate granule-associated protein PhaI PP_5008 +0.1
polyhydroxyalkanoate synthesis repressor PhaR No genes

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