SEED Subsystem: Arginine and Ornithine Degradation in Escherichia coli BW25113

Experiments:

Role Gene Name Outgrowth from conjugation with pRH23 in LB+Gent50  
Agmatinase (EC 3.5.3.11) b2937 speB -0.9
Agmatine deiminase (EC 3.5.3.12) No genes
Arginase (EC 3.5.3.1) No genes
Arginine ABC transporter, ATP-binding protein ArtP b0864 artP -0.0
Arginine ABC transporter, periplasmic arginine-binding protein ArtI b0863 artI +0.2
Arginine ABC transporter, periplasmic arginine-binding protein ArtJ b0860 artJ -0.1
Arginine ABC transporter, permease protein ArtM b0861 artM -0.4
Arginine ABC transporter, permease protein ArtQ b0862 artQ -0.1
Arginine N-succinyltransferase (EC 2.3.1.109) b1747 astA -1.3
Arginine N-succinyltransferase, alpha subunit (EC 2.3.1.109) No genes
Arginine N-succinyltransferase, beta subunit (EC 2.3.1.109) No genes
Arginine decarboxylase (EC 4.1.1.19) No genes
Arginine decarboxylase, catabolic (EC 4.1.1.19) b4117 adiA -0.0
Arginine deiminase (EC 3.5.3.6) No genes
Arginine pathway regulatory protein ArgR, repressor of arg regulon b3237 argR -1.6
Arginine permease RocE No genes
Arginine utilization protein RocB No genes
Arginine utilization regulatory protein RocR No genes
Arginine/agmatine antiporter b4115 adiC -0.1
Arginine/ornithine ABC transporter, ATP-binding protein AotP No genes
Arginine/ornithine ABC transporter, periplasmic arginine/ornithine binding protein No genes
Arginine/ornithine ABC transporter, permease protein AotM No genes
Arginine/ornithine ABC transporter, permease protein AotQ No genes
Arginine/ornithine antiporter ArcD b1605 ydgI -0.2
Biosynthetic arginine decarboxylase (EC 4.1.1.19) b2938 speA -0.3
Carbamate kinase (EC 2.7.2.2) b0323 yahI +0.1
  b0521 ybcF +0.2
Delta-1-pyrroline-5-carboxylate dehydrogenase (EC 1.5.1.12) No genes
Histidine ABC transporter, ATP-binding protein HisP (TC 3.A.1.3.1) b2306 hisP +0.1
Histidine ABC transporter, histidine-binding periplasmic protein precursor HisJ (TC 3.A.1.3.1) b2309 hisJ -0.0
Histidine ABC transporter, permease protein HisM (TC 3.A.1.3.1) b2307 hisM +0.2
Histidine ABC transporter, permease protein HisQ (TC 3.A.1.3.1) b2308 hisQ +0.0
Lysine-arginine-ornithine-binding periplasmic protein precursor (TC 3.A.1.3.1) b2310 argT +0.1
N-carbamoylputrescine amidase (3.5.1.53) No genes
NADP-specific glutamate dehydrogenase (EC 1.4.1.4) b1761 gdhA +0.2
Ornithine aminotransferase (EC 2.6.1.13) No genes
Ornithine carbamoyltransferase (EC 2.1.3.3) b0273 argF +0.1
  b4254 argI +0.2
Ornithine cyclodeaminase (EC 4.3.1.12) No genes
Ornithine decarboxylase (EC 4.1.1.17) b0693 speF -0.0
  b2965 speC +0.2
Putrescine aminotransferase (EC 2.6.1.82) b3073 ygjG +0.1
Succinylarginine dihydrolase (EC 3.5.3.23) b1745 astB -1.2
Succinylglutamate desuccinylase (EC 3.5.1.96) b1744 astE -1.2
Succinylglutamic semialdehyde dehydrogenase (EC 1.2.1.71) b1746 astD -1.1
Succinylornithine transaminase (EC 2.6.1.81) b1748 astC -1.5
Transcriptional regulator ArcR essential for anaerobic expression of the ADI pathway, Crp/Fnr family No genes
Transcriptional regulator of catabolic arginine decarboxylase (adiA) b0566 envY +0.0
  b4116 adiY -0.1

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