SEED Subsystem: Serine-glyoxylate cycle in Escherichia coli BW25113

Experiments:

Role Gene Name vcDART vector transformation; liquid outgrowth assay  
⨯
3-ketoacyl-CoA thiolase (EC 2.3.1.16) No genes
5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20) b3941 metF -0.0
5-FCL-like protein, but predicted not to be 5-formyltetrahydrofolate cyclo-ligase (5-FCL) No genes
5-formyltetrahydrofolate cyclo-ligase (EC 6.3.3.2) b2912 ygfA -0.7
Acetoacetyl-CoA reductase (EC 1.1.1.36) No genes
Acetyl-CoA acetyltransferase (EC 2.3.1.9) No genes
Aconitate hydratase (EC 4.2.1.3) No genes
Citrate synthase (EC 2.3.3.1) No genes
Citrate synthase (si) (EC 2.3.3.1) b0720 gltA +3.9
Enolase (EC 4.2.1.11) b2779 eno N.D.
Formate--tetrahydrofolate ligase (EC 6.3.4.3) No genes
Formiminotetrahydrofolate cyclodeaminase (EC 4.3.1.4) No genes
Fumarate hydratase class I, aerobic (EC 4.2.1.2) b1612 fumA +2.8
Fumarate hydratase class I, anaerobic (EC 4.2.1.2) b4122 fumB +0.0
GDSL-like lipase/acylhydrolase No genes
Glycerate kinase (EC 2.7.1.31) b0514 glxK +0.4
Isocitrate lyase (EC 4.1.3.1) b4015 aceA +0.0
Malate dehydrogenase (EC 1.1.1.37) b0801 ybiC -0.4
  b3236 mdh +2.0
Malate synthase-related protein No genes
Malyl-CoA lyase (EC 4.1.3.24) No genes
Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9) b0529 folD N.D.
Methylcrotonyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.4) No genes
Methylcrotonyl-CoA carboxylase carboxyl transferase subunit (EC 6.4.1.4) No genes
Methylene tetrahydromethanopterin dehydrogenase (EC 1.5.99.9) No genes
Methylenetetrahydrofolate dehydrogenase (NADP+) (EC 1.5.1.5) b0529 folD N.D.
Methylmalonyl-CoA mutase (EC 5.4.99.2) b2917 yliK +0.1
N(5),N(10)-methenyltetrahydromethanopterin cyclohydrolase (EC 3.5.4.27) No genes
Phosphoenolpyruvate carboxykinase [ATP] (EC 4.1.1.49) b3403 pck -2.4
Phosphoglyceromutase No genes
Propionyl-CoA carboxylase beta chain (EC 6.4.1.3) No genes
Propionyl-CoA carboxylase carboxyl transferase subunit (EC 6.4.1.3) No genes
Putative malate dehydrogenase (EC 1.1.1.37), similar to archaeal MJ1425 No genes
Serine hydroxymethyltransferase (EC 2.1.2.1) b2551 glyA N.D.
Serine--glyoxylate aminotransferase (EC 2.6.1.45) No genes
Serine--pyruvate aminotransferase (EC 2.6.1.51) No genes
Serine-pyruvate aminotransferase/archaeal aspartate aminotransferase No genes
Succinate dehydrogenase flavoprotein subunit (EC 1.3.99.1) b0723 sdhA +0.9
  b4154 frdA -0.0
Succinate dehydrogenase iron-sulfur protein (EC 1.3.99.1) b0724 sdhB +1.1
  b4153 frdB +0.1
Succinyl-CoA ligase [ADP-forming] alpha chain (EC 6.2.1.5) b0729 sucD +0.4
Succinyl-CoA ligase [ADP-forming] beta chain (EC 6.2.1.5) b0728 sucC -1.3
Succinyl-CoA:3-ketoacid-coenzyme A transferase subunit A (EC 2.8.3.5) No genes
Succinyl-CoA:3-ketoacid-coenzyme A transferase subunit B (EC 2.8.3.5) No genes
crotonyl-CoA reductase No genes
cytosolic long-chain acyl-CoA thioester hydrolase family protein No genes
decarboxylase No genes
isobutyryl-CoA dehydrogenase No genes
low-specificity D-threonine aldolase No genes
polyhydroxyalkanoate synthesis repressor PhaR No genes
putative glycerate kinase No genes
putative isobutyryl-CoA mutase, chain B No genes

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