SEED Subsystem: TCA Cycle in Escherichia coli BW25113

Experiments:

Role Gene Name vcDART vector transformation; agar assay  
⨯
2-oxoglutarate dehydrogenase E1 component (EC 1.2.4.2) b0726 sucA +0.6
Aconitate hydratase (EC 4.2.1.3) No genes
Aconitate hydratase 2 (EC 4.2.1.3) No genes
Aconitate hydratase X, predicted No genes
Aconitate hydratase large subunit (EC 4.2.1.3) No genes
Aconitate hydratase small subunit (EC 4.2.1.3) No genes
Archaeal succinyl-CoA ligase [ADP-forming] alpha chain (EC 6.2.1.5) No genes
Archaeal succinyl-CoA ligase [ADP-forming] beta chain (EC 6.2.1.5) No genes
Citrate lyase gamma chain, acyl carrier protein (EC 4.1.3.6) b0617 citD -0.1
Citrate synthase (si) (EC 2.3.3.1) b0720 gltA +3.5
Dihydrolipoamide dehydrogenase (EC 1.8.1.4) No genes
Dihydrolipoamide dehydrogenase of 2-oxoglutarate dehydrogenase (EC 1.8.1.4) No genes
Dihydrolipoamide dehydrogenase of pyruvate dehydrogenase complex (EC 1.8.1.4) No genes
Dihydrolipoamide succinyltransferase component (E2) of 2-oxoglutarate dehydrogenase complex (EC 2.3.1.61) b0727 sucB N.D.
Fumarate hydratase class I (EC 4.2.1.2) No genes
Fumarate hydratase class I, aerobic (EC 4.2.1.2) b1612 fumA +1.8
Fumarate hydratase class II (EC 4.2.1.2) b1611 fumC -0.2
Isocitrate dehydrogenase [NADP] (EC 1.1.1.42) b1136 icd N.D.
Isocitrate dehydrogenase [NAD] (EC 1.1.1.41) No genes
Malate dehydrogenase (EC 1.1.1.37) b0801 ybiC +0.2
  b3236 mdh +2.5
Malate:quinone oxidoreductase (EC 1.1.5.4) b2210 mqo +0.2
Putative Dihydrolipoamide dehydrogenase (EC 1.8.1.4) No genes
Putative malate dehydrogenase (EC 1.1.1.37), similar to archaeal MJ1425 No genes
Succinate dehydrogenase flavoprotein subunit (EC 1.3.99.1) b0723 sdhA +2.2
  b4154 frdA +0.2
Succinate dehydrogenase iron-sulfur protein (EC 1.3.99.1) b0724 sdhB +2.0
  b4153 frdB +0.2
Succinyl-CoA ligase [ADP-forming] alpha chain (EC 6.2.1.5) b0729 sucD +2.0
Succinyl-CoA ligase [ADP-forming] beta chain (EC 6.2.1.5) b0728 sucC +1.3
Transcriptional regulator of succinyl CoA synthetase operon No genes
hypothetical protein that often co-occurs with aconitase No genes

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