SEED Subsystem: Nitrate and nitrite ammonification in Escherichia coli BW25113

Experiments:

Role Gene Name M9 with Methotrexate 1.98 mM 24 hr  
⨯
Assimilatory nitrate reductase large subunit (EC:1.7.99.4) No genes
Cytochrome c nitrite reductase, small subunit NrfH No genes
Cytochrome c-type heme lyase subunit nrfE, nitrite reductase complex assembly b4074 nrfE -0.1
Cytochrome c-type heme lyase subunit nrfF, nitrite reductase complex assembly b4075 nrfF +0.2
Cytochrome c-type heme lyase subunit nrfG, nitrite reductase complex assembly b4076 nrfG +0.3
Cytochrome c-type protein NapC b2202 napC -0.3
Cytochrome c-type protein NrfB precursor b4071 nrfB -0.4
Cytochrome c552 precursor (EC 1.7.2.2) b4070 nrfA +0.0
Ferredoxin--nitrite reductase (EC 1.7.7.1) No genes
Ferredoxin-type protein NapF (periplasmic nitrate reductase) b2208 napF +0.3
Ferredoxin-type protein NapG (periplasmic nitrate reductase) b2205 napG +0.1
Nitrate ABC transporter, ATP-binding protein No genes
Nitrate ABC transporter, nitrate-binding protein No genes
Nitrate ABC transporter, permease protein No genes
Nitrate reductase cytochrome c550-type subunit b2203 napB +0.1
Nitrate/nitrite response regulator protein b2193 narP -0.0
Nitrate/nitrite sensor protein (EC 2.7.3.-) b1222 narX +0.5
  b2469 narQ +2.6
Nitrate/nitrite transporter No genes
Nitrite reductase [NAD(P)H] large subunit (EC 1.7.1.4) b3365 nirB +0.1
Nitrite reductase [NAD(P)H] small subunit (EC 1.7.1.4) b3366 nirD +0.0
Nitrite reductase probable [NAD(P)H] subunit (EC 1.7.1.4) No genes
Nitrite reductase probable electron transfer 4Fe-S subunit (EC 1.7.1.4) No genes
Nitrite transporter NirC b3367 nirC -0.0
Nitrite transporter from formate/nitrite family No genes
NrfC protein b4072 nrfC -0.1
NrfD protein b4073 nrfD +0.0
Periplasmic nitrate reductase component NapD b2207 napD -0.0
Periplasmic nitrate reductase component NapE No genes
Periplasmic nitrate reductase component NapL No genes
Periplasmic nitrate reductase precursor (EC 1.7.99.4) b2206 napA +0.2
Polyferredoxin NapH (periplasmic nitrate reductase) b2204 napH +0.3
Putative thiol:disulfide oxidoreductase, nitrite reductase complex assembly No genes
Respiratory nitrate reductase alpha chain (EC 1.7.99.4) b1224 narG +0.1
  b1468 narZ -0.0
Respiratory nitrate reductase beta chain (EC 1.7.99.4) b1225 narH -0.2
  b1467 narY +0.1
Respiratory nitrate reductase delta chain (EC 1.7.99.4) b1226 narJ +0.0
  b1466 narW +0.2
Respiratory nitrate reductase gamma chain (EC 1.7.99.4) b1227 narI +0.2
  b1465 narV +0.2
Respiratory nitrate reductase subunit, conjectural (EC 1.7.99.4) No genes
Response regulator NasT No genes

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