SEED Subsystem: KDO2-Lipid A biosynthesis in Pseudomonas putida KT2440

Experiments:

Role Gene Name P. put PUT 10 MOI 1  
⨯
2-Keto-3-deoxy-D-manno-octulosonate-8-phosphate synthase (EC 2.5.1.55) PP_1611 -0.1
  PP_1807 -1.5
3-deoxy-D-manno-octulosonate 8-phosphate phosphatase (EC 3.1.3.45) PP_0956 N.D.
3-deoxy-D-manno-octulosonic-acid transferase (EC 2.-.-.-) No genes
3-deoxy-manno-octulosonate cytidylyltransferase (EC 2.7.7.38) PP_1902 N.D.
Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (EC 2.3.1.129) PP_1603 N.D.
Arabinose 5-phosphate isomerase (EC 5.3.1.13) PP_0957 +1.0
  PP_1806 -0.4
LPS-assembly lipoprotein RlpB precursor (Rare lipoprotein B) PP_4795 N.D.
Lipid A biosynthesis (KDO) 2-(lauroyl)-lipid IVA acyltransferase (EC 2.3.1.-) No genes
Lipid A biosynthesis lauroyl acyltransferase (EC 2.3.1.-) PP_0063 +1.3
  PP_1735 N.D.
Lipid A export ATP-binding/permease protein MsbA (EC 3.6.3.25) No genes
Lipid-A-disaccharide synthase (EC 2.4.1.182) PP_1604 N.D.
Lipopolysaccharide ABC transporter, ATP-binding protein LptB PP_0953 N.D.
LptA, protein essential for LPS transport across the periplasm PP_0954 N.D.
O-antigen flippase Wzx No genes
Oligosaccharide repeat unit polymerase Wzy No genes
Predicted hydrolase of the metallo-beta-lactamase superfamily, clustered with KDO2-Lipid A biosynthesis genes No genes
Protein of unknown function DUF1009 clustered with KDO2-Lipid A biosynthesis genes No genes
Tetraacyldisaccharide 4'-kinase (EC 2.7.1.130) PP_1900 N.D.
UDP-2,3-diacylglucosamine hydrolase (EC 3.6.1.-) No genes
UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase (EC 3.5.1.-) No genes
UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase (EC 2.3.1.-) No genes
UDP-N-acetylglucosamine 4-epimerase (EC 5.1.3.7) No genes
regulator of length of O-antigen component of lipopolysaccharide chains No genes

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