SEED Subsystem: Polyhydroxybutyrate metabolism in Pseudomonas putida KT2440

Experiments:

Role Gene Name Ethyl decanoate (C)  
⨯
3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) PP_2047 -0.3
  PP_2136 -3.6
3-hydroxybutyryl-CoA dehydratase (EC 4.2.1.55) No genes
3-hydroxybutyryl-CoA dehydrogenase (EC 1.1.1.157) PP_3755 -0.3
3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3) PP_2047 -0.3
  PP_2136 -3.6
3-ketoacyl-CoA thiolase (EC 2.3.1.16) PP_3355 -0.1
Acetoacetyl-CoA reductase (EC 1.1.1.36) No genes
Acetoacetyl-CoA synthetase (EC 6.2.1.16) PP_3071 +0.0
  PP_3458 +0.2
Acetyl-CoA acetyltransferase (EC 2.3.1.9) PP_4636 +0.0
Butyrate-acetoacetate CoA-transferase subunit A (EC 2.8.3.9) No genes
Butyrate-acetoacetate CoA-transferase subunit B (EC 2.8.3.9) No genes
D(-)-3-hydroxybutyrate oligomer hydrolase (EC 3.1.1.22) No genes
D-beta-hydroxybutyrate dehydrogenase (EC 1.1.1.30) PP_2784 -0.3
  PP_3073 -0.1
D-beta-hydroxybutyrate permease PP_3074 -0.2
Enoyl-CoA hydratase (EC 4.2.1.17) PP_1412 -0.1
  PP_2047 -0.3
  PP_2136 -3.6
  PP_3726 +0.3
  PP_3732 -0.3
Intracellular PHB depolymerase (EC 3.1.1.-) No genes
Poly(3-hydroxyalkanoate) depolymerase PP_5004 -0.3
Polyhydroxyalkanoate granule-associated protein PhaF PP_5007 -0.6
Polyhydroxyalkanoic acid synthase PP_5003 +1.2
  PP_5005 +1.0
Putative 3-hydroxyacyl-coa dehydrogenase No genes
Short chain fatty acids transporter PP_3124 +0.1
polyhydroxyalkanoate granule-associated protein PhaI PP_5008 +0.0
polyhydroxyalkanoate synthesis repressor PhaR No genes

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