SEED Subsystem: Choline and Betaine Uptake and Betaine Biosynthesis in Pseudomonas putida KT2440

Experiments:

Role Gene Name Betaine (N)  
Alcohol dehydrogenase GbsB (type III ), essential for the utilization of choline (EC 1.1.1.1) No genes
Betaine aldehyde dehydrogenase (EC 1.2.1.8) PP_5063 +0.5
Choline ABC transport system, ATP-binding protein OpuBA No genes
Choline ABC transport system, choline-binding protein OpuBC No genes
Choline ABC transport system, permease protein OpuBB No genes
Choline ABC transport system, permease protein OpuBD No genes
Choline binding protein A No genes
Choline binding protein D No genes
Choline dehydrogenase (EC 1.1.99.1) PP_0056 +0.0
  PP_1949 +0.1
  PP_5064 -0.1
Choline-sulfatase (EC 3.1.6.6) PP_0077 -0.1
  PP_3352 +0.1
GbcA Glycine betaine demethylase subunit A PP_0315 -1.2
GbcB Glycine betaine demethylase subunit B PP_0316 -0.9
Glycine betaine ABC transport system permease protein No genes
Glycine betaine ABC transport system, ATP-binding protein OpuAA (EC 3.6.3.32) No genes
Glycine betaine ABC transport system, glycine betaine-binding protein OpuAC No genes
Glycine betaine ABC transport system, permease protein OpuAB No genes
Glycine betaine ABC transport system, permease/glycine betaine-binding protein OpuABC No genes
Glycine betaine transporter OpuD PP_3628 -0.0
Glycine betaine/L-proline ABC transporter, glycine betaine/L-proline- binding/permease protein PP_1741 +0.2
HTH-type transcriptional regulator BetI PP_5719 +0.0
High-affinity choline uptake protein BetT PP_0229 -0.1
  PP_2692 -0.0
  PP_3957 -0.2
  PP_5061 +0.0
  PP_5374 -0.0
L-proline glycine betaine ABC transport system permease protein ProV (TC 3.A.1.12.1) PP_0294 -0.2
  PP_2774 +0.0
L-proline glycine betaine ABC transport system permease protein ProW (TC 3.A.1.12.1) PP_0295 -0.1
  PP_0869 +0.1
  PP_0871 -0.4
  PP_3559 -0.2
L-proline glycine betaine binding ABC transporter protein ProX (TC 3.A.1.12.1) PP_0076 +0.1
  PP_0296 +0.1
  PP_0870 +0.1
Osmotically activated L-carnitine/choline ABC transporter, ATP-binding protein OpuCA No genes
Osmotically activated L-carnitine/choline ABC transporter, permease protein OpuCB No genes
Osmotically activated L-carnitine/choline ABC transporter, permease protein OpuCD No genes
Osmotically activated L-carnitine/choline ABC transporter, substrate-binding protein OpuCC No genes
Sarcosine oxidase alpha subunit (EC 1.5.3.1) PP_0325 -2.7
Sarcosine oxidase beta subunit (EC 1.5.3.1) PP_0323 -2.6
  PP_2246 +0.0
Sarcosine oxidase delta subunit (EC 1.5.3.1) PP_0324 -3.0
Sarcosine oxidase gamma subunit (EC 1.5.3.1) PP_0326 -2.6

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