SEED Subsystem: Polyhydroxybutyrate metabolism in Pseudomonas simiae WCS417

Experiments:

Role Gene Name Parafilmed volatile agar plate with no fungus  
3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) PS417_07575 +0.0
  PS417_21215 -0.1
3-hydroxybutyryl-CoA dehydratase (EC 4.2.1.55) No genes
3-hydroxybutyryl-CoA dehydrogenase (EC 1.1.1.157) No genes
3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3) PS417_07575 +0.0
  PS417_21215 -0.1
3-ketoacyl-CoA thiolase (EC 2.3.1.16) PS417_13855 -0.3
  PS417_19210 -0.0
Acetoacetyl-CoA reductase (EC 1.1.1.36) No genes
Acetoacetyl-CoA synthetase (EC 6.2.1.16) PS417_12145 -0.4
  PS417_17010 +0.3
  PS417_19290 +0.1
Acetyl-CoA acetyltransferase (EC 2.3.1.9) PS417_10515 +0.1
Butyrate-acetoacetate CoA-transferase subunit A (EC 2.8.3.9) No genes
Butyrate-acetoacetate CoA-transferase subunit B (EC 2.8.3.9) No genes
D(-)-3-hydroxybutyrate oligomer hydrolase (EC 3.1.1.22) No genes
D-beta-hydroxybutyrate dehydrogenase (EC 1.1.1.30) PS417_12150 N.D.
D-beta-hydroxybutyrate permease PS417_12155 +0.1
Enoyl-CoA hydratase (EC 4.2.1.17) PS417_07030 -0.1
  PS417_07575 +0.0
  PS417_08535 -0.0
  PS417_10680 +0.4
  PS417_11445 +3.9
  PS417_22625 -0.1
Intracellular PHB depolymerase (EC 3.1.1.-) No genes
Poly(3-hydroxyalkanoate) depolymerase PS417_01900 -0.1
Polyhydroxyalkanoate granule-associated protein PhaF PS417_01885 +0.1
Polyhydroxyalkanoic acid synthase PS417_01895 +0.2
  PS417_01905 -0.0
Putative 3-hydroxyacyl-coa dehydrogenase No genes
Short chain fatty acids transporter PS417_03330 +0.0
polyhydroxyalkanoate granule-associated protein PhaI PS417_01880 -0.1
polyhydroxyalkanoate synthesis repressor PhaR No genes

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