SEED Subsystem: Branched-Chain Amino Acid Biosynthesis in Pseudomonas simiae WCS417

Experiments:

Role Gene Name Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50  
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(R)-citramalate synthase (EC 2.3.1.182) No genes
2-isopropylmalate synthase (EC 2.3.3.13) PS417_23110 -4.5
3-isopropylmalate dehydratase large subunit (EC 4.2.1.33) PS417_09410 +0.2
  PS417_18600 -8.1
3-isopropylmalate dehydratase small subunit (EC 4.2.1.33) PS417_09415 -0.6
  PS417_18595 -5.8
3-isopropylmalate dehydrogenase (EC 1.1.1.85) PS417_18585 -6.5
Acetolactate synthase large subunit (EC 2.2.1.6) PS417_08225 -1.7
  PS417_10695 +0.4
  PS417_13315 -0.1
  PS417_23810 -4.4
Acetolactate synthase small subunit (EC 2.2.1.6) PS417_23805 -7.2
Acetolactate synthase small subunit (EC 2.2.1.6), Xanthomonadales type No genes
Acetolactate synthase small subunit (EC 2.2.1.6), predicted No genes
Acetolactate synthase small subunit (EC 2.2.1.6), predicted, Archaeal type No genes
Acetolactate synthase small subunit (EC 2.2.1.6), predicted, Archaeal type 2 No genes
Branched-chain amino acid aminotransferase (EC 2.6.1.42) PS417_17565 -1.4
Dihydroxy-acid dehydratase (EC 4.2.1.9) PS417_26890 -3.6
Ketol-acid reductoisomerase (EC 1.1.1.86) PS417_23800 -6.5
Leucine dehydrogenase (EC 1.4.1.9) PS417_05040 -0.1
Leucine-responsive regulatory protein, regulator for leucine (or lrp) regulon and high-affinity branched-chain amino acid transport system PS417_13660 -0.3
  PS417_17610 -0.2
  PS417_23655 -0.1
  PS417_24510 +0.4
  PS417_27710 -3.7
Threonine dehydratase (EC 4.3.1.19) No genes
Threonine dehydratase biosynthetic (EC 4.3.1.19) PS417_12665 -0.3
  PS417_27010 -3.4
Threonine dehydratase, catabolic (EC 4.3.1.19) PS417_12925 -0.4
Valine--pyruvate aminotransferase (EC 2.6.1.66) PS417_23870 -0.1

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