SEED Subsystem: Methionine Biosynthesis in Pseudomonas simiae WCS417

Experiments:

Role Gene Name root sample 6; outgrowth in LB  
⨯
5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20) PS417_26510 -0.8
5-methyltetrahydrofolate--homocysteine methyltransferase (EC 2.1.1.13) PS417_12310 -0.6
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (EC 2.1.1.14) PS417_18775 -0.2
Adenosylhomocysteinase (EC 3.3.1.1) PS417_26505 N.D.
Archaeal S-adenosylmethionine synthetase (EC 2.5.1.6) No genes
Betaine--homocysteine S-methyltransferase (EC 2.1.1.5) No genes
Cystathionine beta-lyase (EC 4.4.1.8) No genes
Cystathionine beta-lyase, type II (EC 4.4.1.8) No genes
Cystathionine beta-synthase (EC 4.2.1.22) No genes
Cystathionine gamma-lyase (EC 4.4.1.1) No genes
Cystathionine gamma-synthase (EC 2.5.1.48) No genes
Cysteine synthase (EC 2.5.1.47) PS417_06550 +0.1
  PS417_07505 +0.1
  PS417_20160 -1.6
  PS417_21045 +2.0
Duplicated ATPase component MtsB of energizing module of methionine-regulated ECF transporter No genes
Homocysteine S-methyltransferase (EC 2.1.1.10) PS417_28185 -1.0
Homoserine O-acetyltransferase (EC 2.3.1.31) PS417_26740 -2.8
  PS417_26745 -1.8
Homoserine O-succinyltransferase (EC 2.3.1.46) No genes
Homoserine dehydrogenase (EC 1.1.1.3) PS417_22885 N.D.
Homoserine kinase (EC 2.7.1.39) PS417_21120 -1.1
  PS417_00395 N.D.
Methionine ABC transporter ATP-binding protein PS417_01065 N.D.
  PS417_25300 N.D.
  PS417_00360 N.D.
Methionine ABC transporter permease protein PS417_01060 +0.7
  PS417_00355 -0.9
Methionine ABC transporter substrate-binding protein PS417_01005 -0.0
  PS417_01070 -0.1
  PS417_00350 +0.2
Methionine repressor MetJ No genes
Methionine synthase activation domain (EC 2.1.1.13) No genes
Methionine transporter MetT No genes
O-acetylhomoserine sulfhydrylase (EC 2.5.1.49) PS417_13420 +0.3
  PS417_18530 -2.8
O-succinylhomoserine sulfhydrylase (EC 2.5.1.48) PS417_18530 -2.8
Predicted regulator of methionine metabolism, ArsR family No genes
S-adenosylhomocysteine nucleosidase (EC 3.2.2.9) No genes
S-adenosylmethionine synthetase (EC 2.5.1.6) PS417_26450 N.D.
S-ribosylhomocysteine lyase (EC 4.4.1.21) No genes
SAM-dependent methlytransferase UbiG No genes
SAM-dependent methlytransferase YrrT No genes
Serine acetyltransferase (EC 2.3.1.30) PS417_01115 +0.3
  PS417_21050 +2.0
  PS417_23210 -0.8
Substrate-specific component MtsA of methionine-regulated ECF transporter No genes
Transcriptional activator MetR PS417_05455 -0.4
Transmembrane component MtsC of energizing module of methionine-regulated ECF transporter No genes

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