MetaCyc Pathway: pyruvate fermentation to hexanol (engineered) in Pseudomonas fluorescens GW456-L13

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Pyruvate synthase:
pyruvate + coenzyme A + 2 an oxidized ferredoxin [iron-sulfur] cluster→acetyl-CoA + CO2 + H+ + 2 a reduced ferredoxin [iron-sulfur] cluster (EC 1.2.7.1)
No genes
Acetyl-CoA C-acyltransferase:
2 acetyl-CoA→acetoacetyl-CoA + coenzyme A (EC 2.3.1.16; 2.3.1.9)
PfGW456L13_2157
PfGW456L13_2411
PfGW456L13_2430
PfGW456L13_2498
PfGW456L13_2538
PfGW456L13_2539
PfGW456L13_29
PfGW456L13_2982
PfGW456L13_3394
PfGW456L13_3408
PfGW456L13_4040
PfGW456L13_4147
PfGW456L13_4590
3-hydroxyacyl-CoA dehydrogenase (in reverse):
acetoacetyl-CoA + NADH + H+→(S)-3-hydroxybutanoyl-CoA + NAD+ (EC 1.1.1.35)
PfGW456L13_1094
PfGW456L13_3395
PfGW456L13_3873
PfGW456L13_4041
PfGW456L13_412
PfGW456L13_4146
(S)-3-hydroxybutanoyl-CoA→crotonyl-CoA + H2O (EC 4.2.1.150)
PfGW456L13_1812
PfGW456L13_2246
PfGW456L13_2412
PfGW456L13_2533
PfGW456L13_2535
PfGW456L13_3210
PfGW456L13_3388
PfGW456L13_3427
PfGW456L13_3636
PfGW456L13_4041
PfGW456L13_412
PfGW456L13_4531
Trans-2-enoyl-CoA reductase (NAD(+)) (in reverse):
crotonyl-CoA + NADH + H+→butanoyl-CoA + NAD+ (EC 1.3.1.44)
PfGW456L13_1630
PfGW456L13_2077
PfGW456L13_2494
PfGW456L13_2536
PfGW456L13_2983
PfGW456L13_2985
PfGW456L13_3209
PfGW456L13_3387
PfGW456L13_3389
PfGW456L13_3460
PfGW456L13_4073
Acetyl-CoA C-acyltransferase:
acetyl-CoA + butanoyl-CoA→3-oxohexanoyl-CoA + coenzyme A (EC 2.3.1.16; 2.3.1.9)
PfGW456L13_2157
PfGW456L13_2411
PfGW456L13_2430
PfGW456L13_2498
PfGW456L13_2538
PfGW456L13_2539
PfGW456L13_29
PfGW456L13_2982
PfGW456L13_3394
PfGW456L13_3408
PfGW456L13_4040
PfGW456L13_4147
PfGW456L13_4590
3-hydroxyacyl-CoA dehydrogenase (in reverse):
3-oxohexanoyl-CoA + NADH + H+→(S)-3-hydroxyhexanoyl-CoA + NAD+ (EC 1.1.1.35)
PfGW456L13_1094
PfGW456L13_3395
PfGW456L13_4041
PfGW456L13_412
PfGW456L13_4146
Long-chain-enoyl-CoA hydratase:
(S)-3-hydroxyhexanoyl-CoA→(2E)-hex-2-enoyl-CoA + H2O (EC 4.2.1.74)
PfGW456L13_1812
PfGW456L13_2246
PfGW456L13_2412
PfGW456L13_2533
PfGW456L13_2535
PfGW456L13_3210
PfGW456L13_3388
PfGW456L13_3427
PfGW456L13_3636
PfGW456L13_4041
PfGW456L13_412
PfGW456L13_4531
Trans-2-enoyl-CoA reductase (NAD(+)) (in reverse):
(2E)-hex-2-enoyl-CoA + NADH + H+→hexanoyl-CoA + NAD+ (EC 1.3.1.44)
No genes
hexanoyl-CoA + NAD(P)H + H+→1-hexanal + coenzyme A + NAD(P)+
No genes
1-hexanal + NADH + H+→hexan-1-ol + NAD+
No genes

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information