MetaCyc Pathway: propanoate fermentation to 2-methylbutanoate in Pseudomonas fluorescens GW456-L13

MetaCyc Pathway: propanoate fermentation to 2-methylbutanoate in Pseudomonas fluorescens GW456-L13

Propionate CoA-transferase:
propanoate + acetyl-CoA→acetate + propanoyl-CoA (EC 2.8.3.1)

No genes

Acetyl-CoA C-acyltransferase:
propanoyl-CoA + acetyl-CoA→2-methylacetoacetyl-CoA + coenzyme A (EC 2.3.1.16)

PfGW456L13_2157

PfGW456L13_2411

PfGW456L13_2430

PfGW456L13_2498

PfGW456L13_2538

PfGW456L13_2539

PfGW456L13_2982

PfGW456L13_3394

PfGW456L13_3408

PfGW456L13_4040

PfGW456L13_4147

PfGW456L13_4590

3-hydroxy-2-methylbutyryl-CoA dehydrogenase (in reverse):
2-methylacetoacetyl-CoA + NADH + H⁺→(2S,3S)-3-hydroxy-2-methylbutanoyl-CoA + NAD⁺ (EC 1.1.1.178)

PfGW456L13_2981

PfGW456L13_4041

PfGW456L13_4146

(2S,3S)-3-hydroxy-2-methylbutanoyl-CoA→(E)-2-methylcrotonoyl-CoA + H₂O (EC 4.2.1.150)

PfGW456L13_1812

PfGW456L13_2246

PfGW456L13_2412

PfGW456L13_2533

PfGW456L13_2535

PfGW456L13_3210

PfGW456L13_3388

PfGW456L13_3427

PfGW456L13_3636

PfGW456L13_4041

PfGW456L13_4146

PfGW456L13_4531

(E)-2-methylcrotonoyl-CoA + a reduced electron-transfer flavoprotein→(2S)-2-methylbutanoyl-CoA + an oxidized electron-transfer flavoprotein + H⁺ (EC 1.3.8.5)

PfGW456L13_2983

PfGW456L13_2985

(2S)-2-methylbutanoyl-CoA + H₂O→2-methylbutanoate + coenzyme A + H⁺

No genes

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information