MetaCyc Pathway: palmitate biosynthesis II (type II fatty acid synthase) in Xanthobacter sp. DMC5

MetaCyc Pathway: palmitate biosynthesis II (type II fatty acid synthase) in Xanthobacter sp. DMC5

3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
an acetoacetyl-[acp] + NADPH + H⁺→a (3R)-3-hydroxybutanoyl-[acp] + NADP⁺ (EC 1.1.1.100)

3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxybutanoyl-[acp]→a (2E)-but-2-enoyl-[acp] + H₂O (EC 4.2.1.59)

Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-but-2-enoyl-[acp] + NADH + H⁺→a butanoyl-[acp] + NAD⁺ (EC 1.3.1.9)

Beta-ketoacyl-acyl-carrier-protein synthase II:
a butanoyl-[acp] + a malonyl-[acp] + H⁺→a 3-oxohexanoyl-[acp] + a soluble [acyl-carrier protein] + CO₂ (EC 2.3.1.179; 2.3.1.41)

3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 3-oxohexanoyl-[acp] + NADPH + H⁺→a (3R)-3-hydroxyhexanoyl-[acp] + NADP⁺ (EC 1.1.1.100)

3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxyhexanoyl-[acp]→a (2E)-hex-2-enoyl-[acp] + H₂O (EC 4.2.1.59)

Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-hex-2-enoyl-[acp] + NADH + H⁺→a hexanoyl-[acp] + NAD⁺ (EC 1.3.1.9)

Beta-ketoacyl-acyl-carrier-protein synthase II:
a hexanoyl-[acp] + a malonyl-[acp] + H⁺→a 3-oxooctanoyl-[acp] + a soluble [acyl-carrier protein] + CO₂ (EC 2.3.1.179; 2.3.1.41)

3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 3-oxooctanoyl-[acp] + NADPH + H⁺→a (3R)-3-hydroxyoctanoyl-[acp] + NADP⁺ (EC 1.1.1.100)

3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxyoctanoyl-[acp]→a (2E)-oct-2-enoyl-[acp] + H₂O (EC 4.2.1.59)

Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-oct-2-enoyl-[acp] + NADH + H⁺→an octanoyl-[acp] + NAD⁺ (EC 1.3.1.9)

Beta-ketoacyl-acyl-carrier-protein synthase II:
an octanoyl-[acp] + a malonyl-[acp] + H⁺→a 3-oxodecanoyl-[acp] + a soluble [acyl-carrier protein] + CO₂ (EC 2.3.1.179; 2.3.1.41)

3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 3-oxodecanoyl-[acp] + NADPH + H⁺→a (3R)-3-hydroxydecanoyl-[acp] + NADP⁺ (EC 1.1.1.100)

3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxydecanoyl-[acp]→a (2E)-dec-2-enoyl-[acp] + H₂O (EC 4.2.1.59)

Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-dec-2-enoyl-[acp] + NADH + H⁺→a decanoyl-[acp] + NAD⁺ (EC 1.3.1.9)

Beta-ketoacyl-acyl-carrier-protein synthase II:
a decanoyl-[acp] + a malonyl-[acp] + H⁺→a 3-oxododecanoyl-[acp] + a soluble [acyl-carrier protein] + CO₂ (EC 2.3.1.179; 2.3.1.41)

3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 3-oxododecanoyl-[acp] + NADPH + H⁺→a (3R)-3-hydroxydodecanoyl-[acp] + NADP⁺ (EC 1.1.1.100)

3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxydodecanoyl-[acp]→a (2E)-dodec-2-enoyl-[acp] + H₂O (EC 4.2.1.59)

Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-dodec-2-enoyl-[acp] + NADH + H⁺→a dodecanoyl-[acp] + NAD⁺ (EC 1.3.1.9)

Dodecanoyl-[acyl-carrier-protein] hydrolase:
a dodecanoyl-[acp]_[nil] + H₂O_[nil]→laurate_[nil] + a soluble [acyl-carrier protein]_[nil] + H⁺_[nil] (EC 3.1.2.21)

No genes

Beta-ketoacyl-acyl-carrier-protein synthase II:
a dodecanoyl-[acp] + a malonyl-[acp] + H⁺→a 3-oxotetradecanoyl-[acp] + a soluble [acyl-carrier protein] + CO₂ (EC 2.3.1.179; 2.3.1.41)

Butyrate--CoA ligase:
laurate + ATP + coenzyme A→lauroyl-CoA + AMP + diphosphate (EC 6.2.1.2; 6.2.1.3)

3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 3-oxotetradecanoyl-[acp] + NADPH + H⁺→a (3R)-3-hydroxytetradecanoyl-[acp] + NADP⁺ (EC 1.1.1.100)

3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxytetradecanoyl-[acp]→a (2E)-tetradec-2-enoyl-[acp] + H₂O (EC 4.2.1.59)

Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-tetradec-2-enoyl-[acp] + NADH + H⁺→a myristoyl-[acp] + NAD⁺ (EC 1.3.1.9)

Beta-ketoacyl-acyl-carrier-protein synthase II:
a myristoyl-[acp] + a malonyl-[acp] + H⁺→a 3-oxohexadecanoyl-[acp] + a soluble [acyl-carrier protein] + CO₂ (EC 2.3.1.179; 2.3.1.41)

3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 3-oxohexadecanoyl-[acp] + NADPH + H⁺→a (3R)-3-hydroxyhexadecanoyl-[acp] + NADP⁺ (EC 1.1.1.100)

3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxyhexadecanoyl-[acp]→a (2E)-hexadec-2-enoyl-[acp] + H₂O (EC 4.2.1.59)

Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-hexadec-2-enoyl-[acp] + NADH + H⁺→a palmitoyl-[acp] + NAD⁺ (EC 1.3.1.9)

Oleoyl-[acyl-carrier-protein] hydrolase:
a palmitoyl-[acp] + H₂O→palmitate + a soluble [acyl-carrier protein] + H⁺ (EC 3.1.2.14)

No genes

Long-chain-fatty-acid--CoA ligase:
palmitate + ATP + coenzyme A→palmitoyl-CoA + AMP + diphosphate (EC 6.2.1.3)

Links:

Pathway details at MetaCyc
Fitness data for 52 genes
Select organism:
All MetaCyc pathways for Xanthobacter sp. DMC5

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information