MetaCyc Pathway: 3-hydroxypropanoate cycle in Xanthobacter sp. DMC5

MetaCyc Pathway: 3-hydroxypropanoate cycle in Xanthobacter sp. DMC5

Carbonate dehydratase (in reverse):
CO₂ + H₂O→hydrogen carbonate + H⁺ (EC 4.2.1.1)

Biotin carboxylase:
hydrogen carbonate + ATP + a [biotin carboxyl-carrier-protein dimer]-N⁶-biotinyl-L-lysine→a [carboxyl-carrier protein dimer]-N⁶-carboxybiotinyl-L-lysine + ADP + H⁺ + phosphate (EC 6.3.4.14)

a [carboxyl-carrier protein dimer]-N⁶-carboxybiotinyl-L-lysine + acetyl-CoA→malonyl-CoA + a [biotin carboxyl-carrier-protein dimer]-N⁶-biotinyl-L-lysine (EC 2.1.3.15)

malonyl-CoA + NADPH + H⁺→3-oxopropanoate + coenzyme A + NADP⁺ (EC 1.2.1.75)

No genes

3-oxopropanoate + NADPH + H⁺→3-hydroxypropanoate + NADP⁺ (EC 1.1.1.298)

3-hydroxypropanoate + ATP + coenzyme A→3-hydroxypropanoyl-CoA + AMP + diphosphate (EC 6.2.1.36)

No genes

3-hydroxypropanoyl-CoA→acryloyl-CoA + H₂O (EC 4.2.1.116)

acryloyl-CoA + NADPH + H⁺→propanoyl-CoA + NADP⁺ (EC 1.3.1.84)

Propionyl-CoA carboxylase:
hydrogen carbonate + propanoyl-CoA + ATP→(S)-methylmalonyl-CoA + ADP + H⁺ + phosphate (EC 6.4.1.3)

Methylmalonyl-CoA epimerase (in reverse):
(S)-methylmalonyl-CoA→(R)-methylmalonyl-CoA (EC 5.1.99.1)

Methylmalonyl-CoA mutase:
(R)-methylmalonyl-CoA→succinyl-CoA (EC 5.4.99.2)

succinyl-CoA + (S)-malate→(S)-malyl-CoA + succinate (EC 2.8.3.22)

No genes

Malyl-CoA lyase:
(S)-malyl-CoA→acetyl-CoA + glyoxylate (EC 4.1.3.24)

Links:

Pathway details at MetaCyc
Fitness data for 20 genes
Select organism:
All MetaCyc pathways for Xanthobacter sp. DMC5

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information