MetaCyc Pathway: L-valine biosynthesis in Pseudomonas simiae WCS417

Acetolactate synthase:
2 pyruvate + H⁺→(S)-2-acetolactate + CO₂ (EC 2.2.1.6)

Ketol-acid reductoisomerase (in reverse):
(S)-2-acetolactate + NADPH + H⁺→(2R)-2,3-dihydroxy-3-methylbutanoate + NADP⁺ (EC 1.1.1.86)

PS417_23800

Dihydroxy-acid dehydratase:
(2R)-2,3-dihydroxy-3-methylbutanoate→3-methyl-2-oxobutanoate + H₂O (EC 4.2.1.9)

PS417_11000

PS417_26890

Branched-chain-amino-acid transaminase (in reverse):
3-methyl-2-oxobutanoate + L-glutamate→L-valine + 2-oxoglutarate (EC 2.6.1.42)

PS417_17565

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Pathway details at MetaCyc
Fitness data for 10 genes
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All MetaCyc pathways for Pseudomonas simiae WCS417

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information