MetaCyc Pathway: superpathway of rosmarinic acid biosynthesis in Pseudomonas simiae WCS417

Add experiment(s):


Phenylalanine ammonia-lyase:
L-phenylalanine→trans-cinnamate + ammonium (EC 4.3.1.24; 4.3.1.25; 5.4.3.10)
No genes
Tyrosine 3-monooxygenase:
L-tyrosine + dioxygen + 5,6,7,8-tetrahydrobiopterin→L-dopa + a 4a-hydroxytetrahydrobiopterin (EC 1.14.16.2)
No genes
Aspartate transaminase:
L-tyrosine + 2-oxoglutarate→3-(4-hydroxyphenyl)pyruvate + L-glutamate (EC 2.6.1.1; 2.6.1.27; 2.6.1.5; 2.6.1.57)
PS417_08130
PS417_08560
PS417_09070
PS417_09120
PS417_11840
PS417_12775
PS417_12985
PS417_13545
PS417_14865
PS417_15040
PS417_15990
PS417_18690
PS417_19025
PS417_19385
PS417_19910
PS417_21030
PS417_21275
PS417_23120
PS417_23365
PS417_27730
PS417_28165
PS417_04425
Hydroxyphenylpyruvate reductase (in reverse):
3-(4-hydroxyphenyl)pyruvate + NAD(P)H + H+→(R)-3-(4-hydroxyphenyl)lactate + NAD(P)+ (EC 1.1.1.237)
PS417_15020
Dihydroxyphenylalanine transaminase:
L-dopa + 2-oxoglutarate→3-(3,4-dihydroxyphenyl)pyruvate + L-glutamate (EC 2.6.1.49)
No genes
trans-cinnamate + dioxygen + a reduced [NADPH-hemoprotein reductase]→4-coumarate + an oxidized [NADPH-hemoprotein reductase] + H2O (EC 1.14.14.91)
No genes
4-coumarate--CoA ligase:
4-coumarate + ATP + coenzyme A→(E)-4-coumaroyl-CoA + AMP + diphosphate (EC 6.2.1.12)
No genes
Hydroxyphenylpyruvate reductase (in reverse):
3-(3,4-dihydroxyphenyl)pyruvate + NAD(P)H + H+→(R)-3-(3,4-dihydroxyphenyl)lactate + NAD(P)+ (EC 1.1.1.237)
PS417_15020
(R)-3-(4-hydroxyphenyl)lactate + (E)-4-coumaroyl-CoA→4-coumaroyl-4'-hydroxyphenyllactate + coenzyme A
No genes
Rosmarinate synthase:
(R)-3-(3,4-dihydroxyphenyl)lactate + (E)-caffeoyl-CoA→(R)-rosmarinate + coenzyme A (EC 2.3.1.140)
No genes
4-coumaroyl-4'-hydroxyphenyllactate + dioxygen + a reduced [NADPH-hemoprotein reductase]→caffeoyl-4'-hydroxyphenyllactate + an oxidized [NADPH-hemoprotein reductase] + H2O
No genes
4-coumaroyl-4'-hydroxyphenyllactate + dioxygen + a reduced [NADPH-hemoprotein reductase]→4-coumaroyl-3',4'-dihydroxyphenyllactate + an oxidized [NADPH-hemoprotein reductase] + H2O
No genes
caffeoyl-4'-hydroxyphenyllactate + dioxygen + a reduced [NADPH-hemoprotein reductase]→(R)-rosmarinate + an oxidized [NADPH-hemoprotein reductase] + H2O
No genes
4-coumaroyl-3',4'-dihydroxyphenyllactate + dioxygen + a reduced [NADPH-hemoprotein reductase]→(R)-rosmarinate + an oxidized [NADPH-hemoprotein reductase] + H2O
No genes

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information