MetaCyc Pathway: L-isoleucine biosynthesis I (from threonine) in Pseudomonas simiae WCS417

Threonine ammonia-lyase: L-threonine→(Z)-2-aminobutenoate + H2O (EC 4.3.1.19)

PS417_12665

PS417_12925

PS417_27010

1-aminocyclopropane-1-carboxylate deaminase: (Z)-2-aminobutenoate→2-iminobutanoate (spontaneous) (EC 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)

PS417_11735

PS417_12665

PS417_12925

PS417_17160

PS417_27010

2-iminobutanoate + H2O→2-oxobutanoate + ammonium (spontaneous) (EC 3.5.99.10; 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)

PS417_11735

PS417_12665

PS417_12925

PS417_17160

PS417_27010

PS417_27835

Acetolactate synthase: 2-oxobutanoate + H+ + pyruvate→(S)-2-aceto-2-hydroxybutanoate + CO2 (EC 2.2.1.6)

PS417_07295

PS417_08225

PS417_10695

PS417_13315

PS417_23805

PS417_23810

Ketol-acid reductoisomerase (in reverse): (S)-2-aceto-2-hydroxybutanoate + NADPH + H+→(R)-2,3-dihydroxy-3-methylpentanoate + NADP+ (EC 1.1.1.86)

PS417_23800

Dihydroxy-acid dehydratase: (R)-2,3-dihydroxy-3-methylpentanoate→(3S)-3-methyl-2-oxopentanoate + H2O (EC 4.2.1.9)

PS417_11000

PS417_26890

Branched-chain-amino-acid transaminase (in reverse): (3S)-3-methyl-2-oxopentanoate + L-glutamate→L-isoleucine + 2-oxoglutarate (EC 2.6.1.42)

PS417_17565

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information