MetaCyc Pathway: ketogenesis in Variovorax sp. SCN45

MetaCyc Pathway: ketogenesis in Variovorax sp. SCN45

Acetyl-CoA C-acyltransferase:
2 acetyl-CoA→acetoacetyl-CoA + coenzyme A (EC 2.3.1.16; 2.3.1.9)

Hydroxymethylglutaryl-CoA synthase:
acetoacetyl-CoA + acetyl-CoA + H₂O→(S)-3-hydroxy-3-methylglutaryl-CoA + coenzyme A + H⁺ (EC 2.3.3.10)

Hydroxymethylglutaryl-CoA lyase:
(S)-3-hydroxy-3-methylglutaryl-CoA→acetyl-CoA + acetoacetate (EC 4.1.3.4)

3-hydroxybutyrate dehydrogenase (in reverse):
acetoacetate + NADH + H⁺→(R)-3-hydroxybutanoate + NAD⁺ (EC 1.1.1.30)

Acetoacetate decarboxylase:
acetoacetate + H⁺→acetone + CO₂ (EC 4.1.1.4)

Links:

Pathway details at MetaCyc
Fitness data for 32 genes
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All MetaCyc pathways for Variovorax sp. SCN45

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information