MetaCyc Pathway: 3-hydroxypropanoate/4-hydroxybutanate cycle in Variovorax sp. SCN45

MetaCyc Pathway: 3-hydroxypropanoate/4-hydroxybutanate cycle in Variovorax sp. SCN45

Carbonate dehydratase (in reverse):
CO₂ + H₂O→hydrogen carbonate + H⁺ (EC 4.2.1.1)

Biotin carboxylase:
hydrogen carbonate + ATP + a [biotin carboxyl-carrier-protein dimer]-N⁶-biotinyl-L-lysine→a [carboxyl-carrier protein dimer]-N⁶-carboxybiotinyl-L-lysine + ADP + H⁺ + phosphate (EC 6.3.4.14)

a [carboxyl-carrier protein dimer]-N⁶-carboxybiotinyl-L-lysine + acetyl-CoA→malonyl-CoA + a [biotin carboxyl-carrier-protein dimer]-N⁶-biotinyl-L-lysine (EC 2.1.3.15)

malonyl-CoA + NADPH + H⁺→3-oxopropanoate + coenzyme A + NADP⁺ (EC 1.2.1.75)

No genes

3-oxopropanoate + NADPH + H⁺→3-hydroxypropanoate + NADP⁺ (EC 1.1.1.298)

3-hydroxypropanoate + ATP + coenzyme A→3-hydroxypropanoyl-CoA + AMP + diphosphate (EC 6.2.1.36)

No genes

3-hydroxypropanoyl-CoA→acryloyl-CoA + H₂O (EC 4.2.1.116)

No genes

acryloyl-CoA + NADPH + H⁺→propanoyl-CoA + NADP⁺ (EC 1.3.1.84)

Propionyl-CoA carboxylase:
hydrogen carbonate + propanoyl-CoA + ATP→(S)-methylmalonyl-CoA + ADP + H⁺ + phosphate (EC 6.4.1.3)

Methylmalonyl-CoA epimerase (in reverse):
(S)-methylmalonyl-CoA→(R)-methylmalonyl-CoA (EC 5.1.99.1)

Methylmalonyl-CoA mutase:
(R)-methylmalonyl-CoA→succinyl-CoA (EC 5.4.99.2)

succinyl-CoA + NADPH + H⁺→succinate semialdehyde + coenzyme A + NADP⁺ (EC 1.2.1.76)

No genes

succinate semialdehyde + NADPH + H⁺→4-hydroxybutanoate + NADP⁺ (EC 1.1.1.co)

No genes

4-hydroxybutanoate + ATP + coenzyme A→4-hydroxybutanoyl-CoA + AMP + diphosphate (EC 6.2.1.40)

No genes

4-hydroxybutanoyl-CoA→crotonyl-CoA + H₂O (EC 4.2.1.120)

No genes

crotonyl-CoA + H₂O→(S)-3-hydroxybutanoyl-CoA (EC 4.2.1.150)

3-hydroxyacyl-CoA dehydrogenase:
(S)-3-hydroxybutanoyl-CoA + NAD⁺→acetoacetyl-CoA + NADH + H⁺ (EC 1.1.1.35)

Acetyl-CoA C-acyltransferase (in reverse):
acetoacetyl-CoA + coenzyme A→2 acetyl-CoA (EC 2.3.1.16; 2.3.1.9)

Links:

Pathway details at MetaCyc
Fitness data for 73 genes
Select organism:
All MetaCyc pathways for Variovorax sp. SCN45

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information