MetaCyc Pathway: 3-phenylpropanoate degradation in Variovorax sp. SCN45

MetaCyc Pathway: 3-phenylpropanoate degradation in Variovorax sp. SCN45

3-phenylpropanoate + ATP + coenzyme A→3-phenylpropanoyl-CoA + AMP + diphosphate

No genes

3-phenylpropanoyl-CoA + an oxidized electron carrier→(E)-cinnamoyl-CoA + a reduced two electron carrier

No genes

Enoyl-CoA hydratase (in reverse):
(E)-cinnamoyl-CoA + H₂O→(S)-3-hydroxy-3-phenylpropanoyl-CoA (EC 4.2.1.17)

3-hydroxyacyl-CoA dehydrogenase:
(S)-3-hydroxy-3-phenylpropanoyl-CoA + NAD⁺→3-oxo-3-phenylpropanoyl-CoA + NADH + H⁺ (EC 1.1.1.35)

Acetyl-CoA C-acyltransferase (in reverse):
3-oxo-3-phenylpropanoyl-CoA + coenzyme A→benzoyl-CoA + acetyl-CoA (EC 2.3.1.16)

Benzoyl-CoA 3-monooxygenase:
benzoyl-CoA + NADPH + dioxygen + H⁺→3-hydroxybenzoyl-CoA + NADP⁺ + H₂O (EC 1.14.13.58)

No genes

3-hydroxybenzoyl-CoA + a reduced two electron carrier + dioxygen→gentisate + an oxidized electron carrier + coenzyme A + H⁺

No genes

Gentisate 1,2-dioxygenase:
gentisate + dioxygen→3-maleylpyruvate + H⁺ (EC 1.13.11.4)

Maleylpyruvate isomerase:
3-maleylpyruvate→3-fumarylpyruvate (EC 5.2.1.4)

No genes

3-fumarylpyruvate + H₂O→fumarate + pyruvate + H⁺ (EC 3.7.1.20)

Links:

Pathway details at MetaCyc
Fitness data for 62 genes
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All MetaCyc pathways for Variovorax sp. SCN45

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information