MetaCyc Pathway: reductive TCA cycle I in Variovorax sp. SCN45

Add experiment(s):


2-oxoglutarate synthase (in reverse):
succinyl-CoA + CO2 + H+ + 2 a reduced ferredoxin [iron-sulfur] cluster→2-oxoglutarate + coenzyme A + 2 an oxidized ferredoxin [iron-sulfur] cluster (EC 1.2.7.3)
GFF1881
GFF1882
Isocitrate dehydrogenase (NADP(+)) (in reverse):
2-oxoglutarate + CO2 + NADPH→D-threo-isocitrate + NADP+ (EC 1.1.1.42)
GFF343
GFF5973
Aconitate hydratase (in reverse):
D-threo-isocitrate→cis-aconitate + H2O (EC 4.2.1.3)
GFF270
GFF3905
GFF3909
Aconitate hydratase (in reverse):
cis-aconitate + H2O→citrate (EC 4.2.1.3)
GFF270
GFF3905
GFF3909
ATP citrate synthase (in reverse):
citrate + ATP + coenzyme A→acetyl-CoA + oxaloacetate + ADP + phosphate (EC 2.3.3.8)
No genes
Pyruvate synthase (in reverse):
acetyl-CoA + CO2 + H+ + 2 a reduced ferredoxin [iron-sulfur] cluster→pyruvate + coenzyme A + 2 an oxidized ferredoxin [iron-sulfur] cluster (EC 1.2.7.1)
No genes
Pyruvate carboxylase:
pyruvate + ATP + hydrogen carbonate→oxaloacetate + ADP + H+ + phosphate (EC 6.4.1.1)
No genes
Malate dehydrogenase (in reverse):
oxaloacetate + NADH + H+→(S)-malate + NAD+ (EC 1.1.1.37; 1.1.1.38)
GFF1718
GFF3901
GFF414
GFF4621
GFF7263
Fumarate hydratase:
(S)-malate→fumarate + H2O (EC 4.2.1.2)
GFF1583
GFF1584
GFF1887
GFF479
GFF483
Succinate dehydrogenase (ubiquinone):
fumarate[in] + a menaquinol→succinate[in] + a menaquinone (EC 1.3.5.1)
No genes
Succinate--CoA ligase (ADP-forming):
succinate + ATP + coenzyme A→succinyl-CoA + ADP + phosphate (EC 6.2.1.5)
GFF5405
GFF5406

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information