MetaCyc Pathway: biotin biosynthesis I in Variovorax sp. SCN45

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a malonyl-[acp] + S-adenosyl-L-methionine→a malonyl-[acp] methyl ester + S-adenosyl-L-homocysteine (EC 2.1.1.197)
GFF2415
Beta-ketoacyl-acyl-carrier-protein synthase II:
a malonyl-[acp] + a malonyl-[acp] methyl ester + H+→a 3-oxoglutaryl-[acp] methyl ester + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179; 2.3.1.41)
GFF2669
GFF2782
GFF3320
GFF3861
GFF5072
GFF5927
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 3-oxoglutaryl-[acp] methyl ester + NADPH + H+→a (3R)-3-hydroxyglutaryl-[acp] methyl ester + NADP+ (EC 1.1.1.100)
GFF1040
GFF1065
GFF1348
GFF1568
GFF1573
GFF2104
GFF2166
GFF2281
GFF2420
GFF2421
GFF2643
GFF2697
GFF2922
GFF2967
GFF3062
GFF308
GFF3448
GFF3859
GFF3976
GFF3986
GFF4204
GFF4278
GFF4286
GFF4298
GFF4314
GFF4319
GFF4357
GFF4358
GFF4616
GFF4866
GFF4965
GFF4969
GFF4970
GFF4976
GFF5584
GFF6068
GFF6541
GFF6804
GFF6902
GFF7056
GFF7089
GFF7388
GFF84
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxyglutaryl-[acp] methyl ester→a (2E)-enoylglutaryl-[acp] methyl ester + H2O (EC 4.2.1.59)
GFF382
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-enoylglutaryl-[acp] methyl ester + NADH + H+→a glutaryl-[acp] methyl ester + NAD+ (EC 1.3.1.9)
GFF1657
GFF230
GFF4579
GFF6484
Beta-ketoacyl-acyl-carrier-protein synthase II:
a glutaryl-[acp] methyl ester + a malonyl-[acp] + H+→a 3-oxopimeloyl-[acp] methyl ester + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179; 2.3.1.41)
GFF2669
GFF2782
GFF3320
GFF3861
GFF5072
GFF5927
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 3-oxopimeloyl-[acp] methyl ester + NADPH + H+→a (3R)-3-hydroxypimeloyl-[acp] methyl ester + NADP+ (EC 1.1.1.100)
GFF1040
GFF1065
GFF1348
GFF1568
GFF1573
GFF2104
GFF2166
GFF2281
GFF2420
GFF2421
GFF2643
GFF2697
GFF2922
GFF2967
GFF3062
GFF308
GFF3448
GFF3859
GFF3976
GFF3986
GFF4204
GFF4278
GFF4286
GFF4298
GFF4314
GFF4319
GFF4357
GFF4358
GFF4616
GFF4866
GFF4965
GFF4969
GFF4970
GFF4976
GFF5584
GFF6068
GFF6541
GFF6804
GFF6902
GFF7056
GFF7089
GFF7388
GFF84
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxypimeloyl-[acp] methyl ester→a (2E)-enoylpimeloyl-[acp] methyl ester + H2O (EC 4.2.1.59)
GFF382
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-enoylpimeloyl-[acp] methyl ester + NADH + H+→a pimeloyl-[acp] methyl ester + NAD+ (EC 1.3.1.9)
GFF1657
GFF230
GFF4579
GFF6484
a pimeloyl-[acp] methyl ester + H2O→a pimeloyl-[acp] + methanol + H+ (EC 3.1.1.85)
No genes
8-amino-7-oxononanoate synthase:
a pimeloyl-[acp] + L-alanine + H+→(8S)-8-amino-7-oxononanoate + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.47)
GFF144
Adenosylmethionine--8-amino-7-oxononanoate transaminase:
(8S)-8-amino-7-oxononanoate + S-adenosyl-L-methionine→(7R,8S)-diaminononanoate + S-adenosyl-4-methylsulfanyl-2-oxobutanoate (EC 2.6.1.62)
GFF145
GFF1608
GFF4929
GFF6653
Dethiobiotin synthase:
(7R,8S)-diaminononanoate + ATP + CO2→dethiobiotin + ADP + 3 H+ + phosphate (EC 6.3.3.3)
GFF143
Biotin synthase:
dethiobiotin + H+ + 2 a reduced [2Fe-2S] ferredoxin + S-adenosyl-L-methionine + a sulfurated [sulfur carrier]→4,5-secobiotin + 5'-deoxyadenosine + L-methionine + 2 an oxidized [2Fe-2S] ferredoxin + an unsulfurated [sulfur carrier] (EC 2.8.1.6)
GFF937
Biotin synthase:
4,5-secobiotin + S-adenosyl-L-methionine→biotin + 5'-deoxyadenosine + L-methionine + H+ (EC 2.8.1.6)
GFF937

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information