MetaCyc Pathway: L-valine degradation I in Variovorax sp. OAS795

MetaCyc Pathway: L-valine degradation I in Variovorax sp. OAS795

Branched-chain-amino-acid transaminase:
L-valine + 2-oxoglutarate→3-methyl-2-oxobutanoate + L-glutamate (EC 2.6.1.42)

2-oxoisovalerate dehydrogenase (acylating):
3-methyl-2-oxobutanoate + coenzyme A + NAD⁺→isobutanoyl-CoA + CO₂ + NADH (EC 1.2.1.25)

No genes

isobutanoyl-CoA + an oxidized electron-transfer flavoprotein + H⁺→methylacrylyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.5)

No genes

methylacrylyl-CoA + H₂O→(S)-3-hydroxy-isobutanoyl-CoA (EC 4.2.1.150)

3-hydroxyisobutyryl-CoA hydrolase:
(S)-3-hydroxy-isobutanoyl-CoA + H₂O→(S)-3-hydroxy-isobutanoate + coenzyme A + H⁺ (EC 3.1.2.4)

3-hydroxyisobutyrate dehydrogenase:
(S)-3-hydroxy-isobutanoate + NAD⁺→(S)-methylmalonate-semialdehyde + NADH + H⁺ (EC 1.1.1.31)

(S)-3-amino-2-methylpropionate transaminase (in reverse):
(S)-methylmalonate-semialdehyde + L-glutamate→(S)-3-amino-2-methylpropanoate + 2-oxoglutarate (EC 2.6.1.22)

Methylmalonate-semialdehyde dehydrogenase (acylating):
(S)-methylmalonate-semialdehyde + coenzyme A + NAD⁺ + H₂O→propanoyl-CoA + hydrogen carbonate + NADH + H⁺ (EC 1.2.1.27)

Links:

Pathway details at MetaCyc
Fitness data for 41 genes
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All MetaCyc pathways for Variovorax sp. OAS795

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information