MetaCyc Pathway: TCA cycle I (prokaryotic) in Variovorax sp. OAS795

MetaCyc Pathway: TCA cycle I (prokaryotic) in Variovorax sp. OAS795

2-oxoglutarate + coenzyme A + NAD⁺→succinyl-CoA + CO₂ + NADH (EC 1.2.1.105)

Succinate--CoA ligase (ADP-forming) (in reverse):
succinyl-CoA + ADP + phosphate→succinate + ATP + coenzyme A (EC 6.2.1.5)

Succinate dehydrogenase (ubiquinone):
succinate_[in] + an electron-transfer quinone→fumarate_[in] + an electron-transfer quinol (EC 1.3.5.1)

Fumarate hydratase (in reverse):
fumarate + H₂O→(S)-malate (EC 4.2.1.2)

Malate dehydrogenase:
(S)-malate + NAD⁺→oxaloacetate + NADH + H⁺ (EC 1.1.1.37; 1.1.1.38)

(S)-malate_[in] + an electron-transfer quinone→oxaloacetate_[in] + an electron-transfer quinol (EC 1.1.5.4)

No genes

Citrate (Si)-synthase:
oxaloacetate + acetyl-CoA + H₂O→citrate + coenzyme A + H⁺ (EC 2.3.3.1; 2.3.3.16; 2.3.3.3)

Aconitate hydratase:
citrate→cis-aconitate + H₂O (EC 4.2.1.3)

Aconitate hydratase:
cis-aconitate + H₂O→D-threo-isocitrate (EC 4.2.1.3)

Isocitrate dehydrogenase (NADP(+)):
D-threo-isocitrate + NADP⁺→2-oxoglutarate + CO₂ + NADPH (EC 1.1.1.42)

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Pathway details at MetaCyc
Fitness data for 21 genes
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All MetaCyc pathways for Variovorax sp. OAS795

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information