MetaCyc Pathway: curacin A biosynthesis in Variovorax sp. OAS795

Add experiment(s):


malonyl-CoA + H+→acetyl-CoA + CO2 (EC 2.3.1.235; 4.1.1.9)
ABID97_RS17480
L-cysteine + ATP + a holo-[L-cysteinyl-carrier protein]→an L-cysteinyl-[L-cysteinyl-carrier protein] + AMP + diphosphate (EC 6.2.1.69)
No genes
malonyl-CoA + holo ACPI domain within [CurA polyketide synthase]→malonyl-[ACPI domain within CurA polyketide synthase] + coenzyme A
No genes
malonyl-CoA + holo [CurB acyl-carrier protein]→malonyl-[CurB acyl-carrier protein] + coenzyme A
No genes
acetyl-CoA + holo ACPL domain within [CurA pks]→acetyl-ACPL domain within [CurA pks] + coenzyme A
No genes
malonyl-[CurB acyl-carrier protein] + H+→acetyl-[CurB acyl-carrier protein] + CO2
No genes
acetyl-ACPL domain within [CurA pks] + malonyl-[ACPI domain within CurA polyketide synthase] + H+→acetoacetyl-[ACPI domain within CurA polyketide synthase] + CO2 + holo ACPL domain within [CurA pks]
No genes
acetoacetyl-[ACPI domain within CurA polyketide synthase] + acetyl-[CurB acyl-carrier protein] + H2O→(S)-3-hydroxy-3-methylglutaryl-[ACPI domain within CurA polyketide synthase] + holo [CurB acyl-carrier protein] + H+ (EC 2.3.3.g)
No genes
(S)-3-hydroxy-3-methylglutaryl-[ACPI domain within CurA polyketide synthase] + 2-oxoglutarate + chloride + dioxygen + H+→(S)-4-chloro-3-hydroxy-3-methylglutaryl-[ACPI domain within CurA polyketide synthase] + CO2 + succinate + H2O
No genes
(S)-4-chloro-3-hydroxy-3-methylglutaryl-[ACPI domain within CurA polyketide synthase]→4-chloro-3-methylpent-2-enedioyl-[ACPI domain within CurA polyketide synthase] + H2O (EC 4.2.1.ca)
No genes
4-chloro-3-methylpent-2-enedioyl-[ACPI domain within CurA polyketide synthase] + H+→4-chloro-3-methylbut-2-enoyl-[ACPI domain within CurA polyketide synthase] + CO2 (EC 4.1.1.ai)
No genes
4-chloro-3-methylbut-2-enoyl-[ACPI domain within CurA polyketide synthase] + NADPH→(1R,2S)-2-methylcyclopropane-1-carboxyl-[ACPI domain within CurA polyketide synthase] + chloride + NADP+
No genes
(1R,2S)-2-methylcyclopropane-1-carboxyl-[ACPI domain within CurA polyketide synthase] + an L-cysteinyl-[L-cysteinyl-carrier protein]→a 2-[(1R,2S)-2-methylcyclopropyl]-4,5-dihydro-1,3-thiazole-4-carboxyl--[L-cysteinyl-carrier protein] + holo ACPI domain within [CurA polyketide synthase] + H+ + H2O
No genes
malonyl-CoA + a 2-[(1R,2S)-2-methylcyclopropyl]-4,5-dihydro-1,3-thiazole-4-carboxyl--[L-cysteinyl-carrier protein] + a holo acp domain within [CurG polyketide synthase] + NADPH + 2 H+→curacin intermediate-[CurG polyketide synthase] + CO2 + coenzyme A + NADP+ + a holo-[L-cysteinyl-carrier protein]
No genes
curacin intermediate-[CurG polyketide synthase] + malonyl-CoA + a holo acp domain within [CurH polyketide synthase] + 2 NADPH + 3 H+→curacin intermediate-[CurH polyketide synthase] + CO2 + coenzyme A + a holo acp domain within [CurG polyketide synthase] + 2 NADP+ + 2 H2O
No genes
curacin intermediate-[CurH polyketide synthase] + malonyl-CoA + a holo acp domain within [CurI polyketide synthase] + NADPH + 2 H+→curacin intermediate-[CurI polyketide synthase] + CO2 + coenzyme A + a holo acp domain within [CurH polyketide synthase] + NADP+
No genes
curacin intermediate-[CurI polyketide synthase] + malonyl-CoA + a holo acp domain within [CurJ polyketide synthase] + NADPH + H+ + S-adenosyl-L-methionine→curacin intermediate-[CurJ polyketide synthase] + S-adenosyl-L-homocysteine + CO2 + coenzyme A + a holo acp domain within [CurI polyketide synthase] + NADP+ + 2 H2O
No genes
curacin intermediate-[CurJ polyketide synthase] + malonyl-CoA + a holo acp domain within [CurK polyketide synthase] + 2 NADPH + 3 H+→curacin intermediate-[CurK polyketide synthase] + CO2 + coenzyme A + a holo acp domain within [CurJ polyketide synthase] + 2 NADP+ + H2O
No genes
curacin intermediate-[CurK polyketide synthase] + malonyl-CoA + a holo acp domain within [CurL polyketide synthase] + NADPH + H+ + S-adenosyl-L-methionine→curacin intermediate-[CurL polyketide synthase] + S-adenosyl-L-homocysteine + CO2 + coenzyme A + a holo acp domain within [CurK polyketide synthase] + NADP+
No genes
curacin intermediate-[CurL polyketide synthase] + malonyl-CoA + a holo acp domain within [CurM polyketide synthase] + NADPH + 2 H+→curacin intermediate-[CurM polyketide synthase] + CO2 + coenzyme A + a holo acp domain within [CurL polyketide synthase] + NADP+
No genes
curacin intermediate-[CurM polyketide synthase] + 3'-phosphoadenylyl-sulfate→sulfated curacin intermediate-[CurM polyketide synthase] + adenosine 3',5'-bisphosphate + H+
No genes
sulfated curacin intermediate-[CurM polyketide synthase] + H2O→curacin A + CO2 + a holo acp domain within [CurM polyketide synthase] + H+ + sulfate
No genes

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information