MetaCyc Pathway: adipate biosynthesis in Variovorax sp. OAS795

MetaCyc Pathway: adipate biosynthesis in Variovorax sp. OAS795

3-oxoadipyl-CoA thiolase:
acetyl-CoA + succinyl-CoA→3-oxoadipoyl-CoA + coenzyme A (EC 2.3.1.174)

3-hydroxyacyl-CoA dehydrogenase (in reverse):
3-oxoadipoyl-CoA + NADH + H⁺→(3S)-hydroxyadipyl-CoA + NAD⁺ (EC 1.1.1.35)

Enoyl-CoA hydratase:
(3S)-hydroxyadipyl-CoA→(2E)-5-carboxy-2-pentenoyl-CoA + H₂O (EC 4.2.1.17)

(2E)-5-carboxy-2-pentenoyl-CoA + FADH₂→adipoyl-CoA + FAD + H⁺

Succinate--CoA ligase (ADP-forming) (in reverse):
adipoyl-CoA + ADP + phosphate→adipate + ATP + coenzyme A (EC 6.2.1.5)

Links:

Pathway details at MetaCyc
Fitness data for 46 genes
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All MetaCyc pathways for Variovorax sp. OAS795

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information