MetaCyc Pathway: 2-methyl-branched fatty acid β-oxidation in Variovorax sp. OAS795

MetaCyc Pathway: 2-methyl-branched fatty acid β-oxidation in Variovorax sp. OAS795

Butyrate--CoA ligase:
a short-chain 2-methyl-branched fatty acid + ATP + coenzyme A→a short-chain 2-methyl fatty acyl-CoA + AMP + diphosphate (EC 6.2.1.2)

No genes

a long-chain 2-methyl fatty acyl-CoA + an oxidized electron-transfer flavoprotein + H⁺→a long-chain (2E)-2-methyl-2-enoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.8)

Butyrate--CoA ligase:
a medium-chain 2-methyl-branched fatty acid + ATP + coenzyme A→a medium-chain 2-methyl fatty acyl-CoA + AMP + diphosphate (EC 6.2.1.2)

No genes

a short-chain 2-methyl fatty acyl-CoA + an oxidized electron-transfer flavoprotein + H⁺→a short-chain (2E)-2-methyl-2-enoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.5)

No genes

a medium-chain 2-methyl fatty acyl-CoA + an oxidized electron-transfer flavoprotein + H⁺→a medium-chain (2E)-2-methyl-2-enoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.7)

Enoyl-CoA hydratase (in reverse):
a long-chain (2E)-2-methyl-2-enoyl-CoA + H₂O→a long-chain (3S)-3-hydroxy-2-methylacyl-CoA (EC 4.2.1.17)

Enoyl-CoA hydratase (in reverse):
a short-chain (2E)-2-methyl-2-enoyl-CoA + H₂O→a short-chain (3S)-3-hydroxy-2-methylacyl-CoA (EC 4.2.1.17)

Enoyl-CoA hydratase (in reverse):
a medium-chain (2E)-2-methyl-2-enoyl-CoA + H₂O→a medium-chain (3S)-3-hydroxy-2-methylacyl-CoA (EC 4.2.1.17)

3-hydroxyacyl-CoA dehydrogenase:
a long-chain (3S)-3-hydroxy-2-methylacyl-CoA + NAD⁺→a long-chain 2-methyl-3-oxoacyl-CoA + NADH + H⁺ (EC 1.1.1.35)

3-hydroxy-2-methylbutyryl-CoA dehydrogenase:
a short-chain (3S)-3-hydroxy-2-methylacyl-CoA + NAD⁺→a short-chain 2-methyl-3-oxoacyl-CoA + NADH + H⁺ (EC 1.1.1.178)

No genes

3-hydroxyacyl-CoA dehydrogenase:
a medium-chain (3S)-3-hydroxy-2-methylacyl-CoA + NAD⁺→a medium-chain 2-methyl-3-oxoacyl-CoA + NADH + H⁺ (EC 1.1.1.35)

Acetyl-CoA C-acyltransferase (in reverse):
a long-chain 2-methyl-3-oxoacyl-CoA + coenzyme A→a long-chain 2-methyl fatty acyl-CoA + propanoyl-CoA (EC 2.3.1.16)

Acetyl-CoA C-acyltransferase (in reverse):
a short-chain 2-methyl-3-oxoacyl-CoA + coenzyme A→a short-chain 2-methyl fatty acyl-CoA + propanoyl-CoA (EC 2.3.1.16)

Acetyl-CoA C-acyltransferase (in reverse):
a medium-chain 2-methyl-3-oxoacyl-CoA + coenzyme A→a medium-chain 2-methyl fatty acyl-CoA + propanoyl-CoA (EC 2.3.1.16)

Links:

Pathway details at MetaCyc
Fitness data for 61 genes
Select organism:
All MetaCyc pathways for Variovorax sp. OAS795

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information