MetaCyc Pathway: jasmonic acid biosynthesis in Variovorax sp. OAS795

Add experiment(s):


Lipoxygenase:
α-linolenate + dioxygen→13(S)-HpOTre
(EC 1.13.11.12)
No genes
Hydroperoxide dehydratase:
13(S)-HpOTre→(9Z,13S,15Z)-12,13-epoxyoctadeca-9,11,15-trienoate + H2O
(EC 4.2.1.92)
No genes
Allene-oxide cyclase:
(9Z,13S,15Z)-12,13-epoxyoctadeca-9,11,15-trienoate→(9S,13S,15Z)-12-oxophyto-10,15-dienoate
(EC 5.3.99.6)
No genes
12-oxophytodienoate reductase (in reverse):
(9S,13S,15Z)-12-oxophyto-10,15-dienoate + NADPH + H+→(9S,13S,15Z)-12-oxo-10,11-dihydrophyto-15-enoate + NADP+
(EC 1.3.1.42)
No genes
(9S,13S,15Z)-12-oxo-10,11-dihydrophyto-15-enoate + ATP + coenzyme A→3-oxo-2-(cis-2'-pentenyl)-cyclopentane-1-octanoyl-CoA + AMP + diphosphate
No genes
Acyl-CoA oxidase:
3-oxo-2-(cis-2'-pentenyl)-cyclopentane-1-octanoyl-CoA + dioxygen→3-oxo-2-(cis-2'-pentenyl)-cyclopentane-1-(E-octa-2-enoyl)-CoA + hydrogen peroxide
(EC 1.3.3.6)
No genes
3-oxo-2-(cis-2'-pentenyl)-cyclopentane-1-(E-octa-2-enoyl)-CoA + H2O→3-oxo-2-(cis-2'-pentenyl)-cyclopentane-1-(3R-hydroxyoctanoyl)-CoA
No genes
3-oxo-2-(cis-2'-pentenyl)-cyclopentane-1-(3R-hydroxyoctanoyl)-CoA + NAD+→3-oxo-2-(cis-2'-pentenyl)-cyclopentane-1-(3-oxooctanoyl)-CoA + NADH + H+
No genes
Acetyl-CoA C-acyltransferase (in reverse):
3-oxo-2-(cis-2'-pentenyl)-cyclopentane-1-(3-oxooctanoyl)-CoA + coenzyme A→3-oxo-2-(cis-2'-pentenyl)-cyclopentane-1-hexanoyl-CoA + acetyl-CoA
(EC 2.3.1.16)
ABID97_RS04675
ABID97_RS05875
ABID97_RS06005
ABID97_RS10975
ABID97_RS11335
ABID97_RS11380
ABID97_RS12420
ABID97_RS15750
ABID97_RS18615
ABID97_RS18775
ABID97_RS20240
ABID97_RS24190
ABID97_RS25195
ABID97_RS26575
ABID97_RS27835
ABID97_RS28230
ABID97_RS28270
ABID97_RS28430
Acyl-CoA oxidase:
3-oxo-2-(cis-2'-pentenyl)-cyclopentane-1-hexanoyl-CoA + dioxygen→3-oxo-2-(cis-2'-pentenyl)-cyclopentane-1-(E-hexa-2-enoyl)-CoA + hydrogen peroxide
(EC 1.3.3.6)
No genes
3-oxo-2-(cis-2'-pentenyl)-cyclopentane-1-(E-hexa-2-enoyl)-CoA + H2O→3-oxo-2-(cis-2'-pentenyl)-cyclopentane-1-(3R-hydroxyhexanoyl)-CoA
No genes
3-oxo-2-(cis-2'-pentenyl)-cyclopentane-1-(3R-hydroxyhexanoyl)-CoA + NAD+→3-oxo-2-(cis-2'-pentenyl)-cyclopentane-1-(3-oxohexanoyl)-CoA + NADH + H+
No genes
Acetyl-CoA C-acyltransferase (in reverse):
3-oxo-2-(cis-2'-pentenyl)-cyclopentane-1-(3-oxohexanoyl)-CoA + coenzyme A→3-oxo-2-(cis-2'-pentenyl)-cyclopentane-1-butanoyl-CoA + acetyl-CoA
(EC 2.3.1.16)
ABID97_RS04675
ABID97_RS05875
ABID97_RS06005
ABID97_RS10975
ABID97_RS11335
ABID97_RS11380
ABID97_RS12420
ABID97_RS15750
ABID97_RS18615
ABID97_RS18775
ABID97_RS20240
ABID97_RS24190
ABID97_RS25195
ABID97_RS26575
ABID97_RS27835
ABID97_RS28230
ABID97_RS28270
ABID97_RS28430
Acyl-CoA oxidase:
3-oxo-2-(cis-2'-pentenyl)-cyclopentane-1-butanoyl-CoA + dioxygen→3-oxo-2-(cis-2'-pentenyl)-cyclopentane-1-(E-buta-2-enoyl)-CoA + hydrogen peroxide
(EC 1.3.3.6)
No genes
3-oxo-2-(cis-2'-pentenyl)-cyclopentane-1-(E-buta-2-enoyl)-CoA + H2O→3-oxo-2-(cis-2'-pentenyl)-cyclopentane-1-(3R-hydroxybutanoyl)-CoA
No genes
3-oxo-2-(cis-2'-pentenyl)-cyclopentane-1-(3R-hydroxybutanoyl)-CoA + NAD+→3-oxo-2-(cis-2'-pentenyl)-cyclopentane-1-(3-oxobutanoyl)-CoA + NADH + H+
No genes
Acetyl-CoA C-acyltransferase (in reverse):
3-oxo-2-(cis-2'-pentenyl)-cyclopentane-1-(3-oxobutanoyl)-CoA + coenzyme A→(+)-7-epi-jasmonoyl-CoA + acetyl-CoA
(EC 2.3.1.16)
ABID97_RS04675
ABID97_RS05875
ABID97_RS06005
ABID97_RS10975
ABID97_RS11335
ABID97_RS11380
ABID97_RS12420
ABID97_RS15750
ABID97_RS18615
ABID97_RS18775
ABID97_RS20240
ABID97_RS24190
ABID97_RS25195
ABID97_RS26575
ABID97_RS27835
ABID97_RS28230
ABID97_RS28270
ABID97_RS28430
Acyl-CoA hydrolase:
(+)-7-epi-jasmonoyl-CoA + H2O→(+)-7-epi-jasmonate + coenzyme A + H+
(EC 3.1.2.20)
ABID97_RS05905
(+)-7-epi-jasmonate→(-)-jasmonate (spontaneous)
No genes

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information