MetaCyc Pathway: pyruvate fermentation to butanol I in Variovorax sp. OAS795

MetaCyc Pathway: pyruvate fermentation to butanol I in Variovorax sp. OAS795

Pyruvate synthase:
pyruvate + coenzyme A + 2 an oxidized ferredoxin [iron-sulfur] cluster→acetyl-CoA + CO₂ + H⁺ + 2 a reduced ferredoxin [iron-sulfur] cluster (EC 1.2.7.1)

No genes

Acetyl-CoA C-acyltransferase:
2 acetyl-CoA→acetoacetyl-CoA + coenzyme A (EC 2.3.1.16; 2.3.1.9)

3-hydroxyacyl-CoA dehydrogenase (in reverse):
acetoacetyl-CoA + NADH + H⁺→(S)-3-hydroxybutanoyl-CoA + NAD⁺ (EC 1.1.1.35)

(S)-3-hydroxybutanoyl-CoA→crotonyl-CoA + H₂O (EC 4.2.1.150)

crotonyl-CoA_[cytosol] + 2 NADH_[cytosol] + 2 an oxidized ferredoxin [iron-sulfur] cluster_[cytosol]→butanoyl-CoA_[cytosol] + 2 NAD⁺_[cytosol] + 2 a reduced ferredoxin [iron-sulfur] cluster_[cytosol] (EC 1.3.1.109)

No genes

Butanal dehydrogenase (in reverse):
butanoyl-CoA + NAD(P)H + H⁺→1-butanal + coenzyme A + NAD(P)⁺ (EC 1.2.1.57)

No genes

Alcohol dehydrogenase (NADP(+)) (in reverse):
1-butanal + NADPH + H⁺→butan-1-ol + NADP⁺ (EC 1.1.1.2)

No genes

1-butanal + NADH + H⁺→butan-1-ol + NAD⁺

No genes

Links:

Pathway details at MetaCyc
Fitness data for 53 genes
Select organism:
All MetaCyc pathways for Variovorax sp. OAS795

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information