MetaCyc Pathway: nitrogen remobilization from senescing leaves in Variovorax sp. OAS795

MetaCyc Pathway: nitrogen remobilization from senescing leaves in Variovorax sp. OAS795

Pyruvate, phosphate dikinase:
pyruvate + ATP + phosphate→phosphoenolpyruvate + AMP + diphosphate + H⁺ (EC 2.7.9.1)

No genes

Phosphoenolpyruvate carboxylase (in reverse):
phosphoenolpyruvate + hydrogen carbonate→oxaloacetate + phosphate (EC 4.1.1.31)

Citrate (Si)-synthase:
oxaloacetate + acetyl-CoA + H₂O→citrate + coenzyme A + H⁺ (EC 2.3.3.1; 2.3.3.16; 2.3.3.3)

Aconitate hydratase:
citrate→cis-aconitate + H₂O (EC 4.2.1.3)

Aconitate hydratase:
cis-aconitate + H₂O→D-threo-isocitrate (EC 4.2.1.3)

Isocitrate dehydrogenase (NADP(+)):
D-threo-isocitrate + NADP⁺→2-oxoglutarate + CO₂ + NADPH (EC 1.1.1.42)

Isocitrate--homoisocitrate dehydrogenase:
D-threo-isocitrate + NAD⁺→2-oxoglutarate + CO₂ + NADH (EC 1.1.1.286; 1.1.1.41)

Glutamate synthase (ferredoxin) (in reverse):
2-oxoglutarate + L-glutamine + 2 H⁺ + 2 a reduced ferredoxin [iron-sulfur] cluster→2 L-glutamate + 2 an oxidized ferredoxin [iron-sulfur] cluster (EC 1.4.7.1)

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Pathway details at MetaCyc
Fitness data for 12 genes
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All MetaCyc pathways for Variovorax sp. OAS795

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information