MetaCyc Pathway: benzoate biosynthesis I (CoA-dependent, β-oxidative) in Variovorax sp. OAS795

MetaCyc Pathway: benzoate biosynthesis I (CoA-dependent, β-oxidative) in Variovorax sp. OAS795

Phenylalanine ammonia-lyase:
L-phenylalanine→trans-cinnamate + ammonium (EC 4.3.1.24; 4.3.1.25; 5.4.3.10)

No genes

trans-cinnamate + ATP + coenzyme A→(E)-cinnamoyl-CoA + AMP + diphosphate

Enoyl-CoA hydratase (in reverse):
(E)-cinnamoyl-CoA + H₂O→(S)-3-hydroxy-3-phenylpropanoyl-CoA (EC 4.2.1.17)

3-hydroxyacyl-CoA dehydrogenase:
(S)-3-hydroxy-3-phenylpropanoyl-CoA + NAD⁺→3-oxo-3-phenylpropanoyl-CoA + NADH + H⁺ (EC 1.1.1.35)

Acetyl-CoA C-acyltransferase (in reverse):
3-oxo-3-phenylpropanoyl-CoA + coenzyme A→benzoyl-CoA + acetyl-CoA (EC 2.3.1.16)

benzoyl-CoA + H₂O→benzoate + coenzyme A + H⁺

No genes

benzoyl-CoA + benzyl alcohol→benzyl benzoate + coenzyme A (EC 2.3.1.196)

No genes

Benzoate--CoA ligase:
benzoate + ATP + coenzyme A→benzoyl-CoA + AMP + diphosphate (EC 6.2.1.25)

benzyl benzoate + H₂O→benzoate + benzyl alcohol + H⁺

No genes

Links:

Pathway details at MetaCyc
Fitness data for 57 genes
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All MetaCyc pathways for Variovorax sp. OAS795

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information