MetaCyc Pathway: superpathway of phenylethylamine degradation in Variovorax sp. OAS795

MetaCyc Pathway: superpathway of phenylethylamine degradation in Variovorax sp. OAS795

Primary-amine oxidase:
2-phenylethylamine + dioxygen + H₂O→phenylacetaldehyde + ammonium + hydrogen peroxide (EC 1.4.3.21; 1.4.3.4)

No genes

Phenylacetaldehyde dehydrogenase:
phenylacetaldehyde + NAD⁺ + H₂O→phenylacetate + NADH + 2 H⁺ (EC 1.2.1.39)

Phenylacetate--CoA ligase:
phenylacetate + ATP + coenzyme A→phenylacetyl-CoA + AMP + diphosphate (EC 6.2.1.30)

phenylacetyl-CoA + NADPH + dioxygen + H⁺→2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA + NADP⁺ + H₂O (EC 1.14.13.149)

2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA→2-oxepin-2(3H)-ylideneacetyl-CoA (EC 5.3.3.18)

2-oxepin-2(3H)-ylideneacetyl-CoA + H₂O→3-oxo-5,6-didehydrosuberyl-CoA semialdehyde (EC 3.3.2.12)

3-oxo-5,6-didehydrosuberyl-CoA semialdehyde + NADP⁺ + H₂O→3-oxo-5,6-didehydrosuberyl-CoA + NADPH + 2 H⁺ (EC 1.2.1.91)

3-oxo-5,6-didehydrosuberyl-CoA + coenzyme A→(2E)-5-carboxy-2-pentenoyl-CoA + acetyl-CoA (EC 2.3.1.223)

No genes

Enoyl-CoA hydratase (in reverse):
(2E)-5-carboxy-2-pentenoyl-CoA + H₂O→(3S)-hydroxyadipyl-CoA (EC 4.2.1.17)

3-hydroxyacyl-CoA dehydrogenase:
(3S)-hydroxyadipyl-CoA + NAD⁺→3-oxoadipoyl-CoA + NADH + H⁺ (EC 1.1.1.35)

3-oxoadipyl-CoA thiolase (in reverse):
3-oxoadipoyl-CoA + coenzyme A→succinyl-CoA + acetyl-CoA (EC 2.3.1.174)

Links:

Pathway details at MetaCyc
Fitness data for 45 genes
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All MetaCyc pathways for Variovorax sp. OAS795

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information