MetaCyc Pathway: 3-hydroxypropanoate/4-hydroxybutanate cycle in Variovorax sp. OAS795

Add experiment(s):


Carbonate dehydratase (in reverse):
CO2 + H2O→hydrogen carbonate + H+ (EC 4.2.1.1)
ABID97_RS25185
Biotin carboxylase:
hydrogen carbonate + ATP + a [biotin carboxyl-carrier-protein dimer]-N6-biotinyl-L-lysine→a [carboxyl-carrier protein dimer]-N6-carboxybiotinyl-L-lysine + ADP + H+ + phosphate (EC 6.3.4.14)
ABID97_RS00115
ABID97_RS04150
ABID97_RS06370
ABID97_RS06375
ABID97_RS12340
ABID97_RS13950
ABID97_RS27370
ABID97_RS27375
a [carboxyl-carrier protein dimer]-N6-carboxybiotinyl-L-lysine + acetyl-CoA→malonyl-CoA + a [biotin carboxyl-carrier-protein dimer]-N6-biotinyl-L-lysine (EC 2.1.3.15)
ABID97_RS08420
ABID97_RS17835
malonyl-CoA + NADPH + H+→3-oxopropanoate + coenzyme A + NADP+ (EC 1.2.1.75)
No genes
3-oxopropanoate + NADPH + H+→3-hydroxypropanoate + NADP+ (EC 1.1.1.298)
No genes
3-hydroxypropanoate + ATP + coenzyme A→3-hydroxypropanoyl-CoA + AMP + diphosphate (EC 6.2.1.36)
No genes
3-hydroxypropanoyl-CoA→acryloyl-CoA + H2O (EC 4.2.1.116)
ABID97_RS26735
acryloyl-CoA + NADPH + H+→propanoyl-CoA + NADP+ (EC 1.3.1.84)
ABID97_RS13275
Propionyl-CoA carboxylase:
hydrogen carbonate + propanoyl-CoA + ATP→(S)-methylmalonyl-CoA + ADP + H+ + phosphate (EC 6.4.1.3)
ABID97_RS08420
ABID97_RS13945
ABID97_RS13950
ABID97_RS17835
Methylmalonyl-CoA epimerase (in reverse):
(S)-methylmalonyl-CoA→(R)-methylmalonyl-CoA (EC 5.1.99.1)
ABID97_RS13960
Methylmalonyl-CoA mutase:
(R)-methylmalonyl-CoA→succinyl-CoA (EC 5.4.99.2)
ABID97_RS13930
succinyl-CoA + NADPH + H+→succinate semialdehyde + coenzyme A + NADP+ (EC 1.2.1.76)
No genes
succinate semialdehyde + NADPH + H+→4-hydroxybutanoate + NADP+ (EC 1.1.1.co)
No genes
4-hydroxybutanoate + ATP + coenzyme A→4-hydroxybutanoyl-CoA + AMP + diphosphate (EC 6.2.1.40)
No genes
4-hydroxybutanoyl-CoA→crotonyl-CoA + H2O (EC 4.2.1.120)
No genes
crotonyl-CoA + H2O→(S)-3-hydroxybutanoyl-CoA (EC 4.2.1.150)
ABID97_RS00815
ABID97_RS01435
ABID97_RS02880
ABID97_RS04670
ABID97_RS05865
ABID97_RS05900
ABID97_RS05990
ABID97_RS06010
ABID97_RS07755
ABID97_RS10620
ABID97_RS15740
ABID97_RS17010
ABID97_RS17470
ABID97_RS18490
ABID97_RS18600
ABID97_RS18605
ABID97_RS18635
ABID97_RS20990
ABID97_RS22770
ABID97_RS24015
ABID97_RS24805
ABID97_RS26735
ABID97_RS27490
ABID97_RS27535
ABID97_RS27555
ABID97_RS27620
ABID97_RS28260
ABID97_RS28410
ABID97_RS29050
3-hydroxyacyl-CoA dehydrogenase:
(S)-3-hydroxybutanoyl-CoA + NAD+→acetoacetyl-CoA + NADH + H+ (EC 1.1.1.35)
ABID97_RS00810
ABID97_RS03030
ABID97_RS05865
ABID97_RS06010
ABID97_RS08805
ABID97_RS10560
ABID97_RS15740
ABID97_RS17005
ABID97_RS18595
Acetyl-CoA C-acyltransferase (in reverse):
acetoacetyl-CoA + coenzyme A→2 acetyl-CoA (EC 2.3.1.16; 2.3.1.9)
ABID97_RS04675
ABID97_RS05875
ABID97_RS06005
ABID97_RS10975
ABID97_RS11335
ABID97_RS11380
ABID97_RS12420
ABID97_RS15750
ABID97_RS18615
ABID97_RS18775
ABID97_RS20240
ABID97_RS24190
ABID97_RS25195
ABID97_RS26575
ABID97_RS27835
ABID97_RS28230
ABID97_RS28270
ABID97_RS28430

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information