MetaCyc Pathway: 2-keto-L-gulonate biosynthesis in Variovorax sp. OAS795

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D-sorbitol dehydrogenase (acceptor):
D-sorbitol + an oxidized electron carrier→keto-L-sorbose + a reduced two electron carrier (EC 1.1.99.21)
ABID97_RS07135
L-sorbose 1-dehydrogenase:
keto-L-sorbose + an oxidized electron carrier→L-sorbosone + a reduced two electron carrier (EC 1.1.99.32)
No genes
L-sorbosone→L-sorbosone 2,6-lactone (spontaneous)
No genes
L-sorbosone 2,6-lactone + NAD+ + H2O→2-keto-L-gulonate + NADH + 2 H+ (EC 1.1.1.M58)
No genes

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information