MetaCyc Pathway: propanoate fermentation to 2-methylbutanoate in Variovorax sp. OAS795

MetaCyc Pathway: propanoate fermentation to 2-methylbutanoate in Variovorax sp. OAS795

Propionate CoA-transferase:
propanoate + acetyl-CoA→acetate + propanoyl-CoA (EC 2.8.3.1)

No genes

Acetyl-CoA C-acyltransferase:
propanoyl-CoA + acetyl-CoA→2-methylacetoacetyl-CoA + coenzyme A (EC 2.3.1.16)

3-hydroxy-2-methylbutyryl-CoA dehydrogenase (in reverse):
2-methylacetoacetyl-CoA + NADH + H⁺→(2S,3S)-3-hydroxy-2-methylbutanoyl-CoA + NAD⁺ (EC 1.1.1.178)

(2S,3S)-3-hydroxy-2-methylbutanoyl-CoA→(E)-2-methylcrotonoyl-CoA + H₂O (EC 4.2.1.150)

(E)-2-methylcrotonoyl-CoA + a reduced electron-transfer flavoprotein→(2S)-2-methylbutanoyl-CoA + an oxidized electron-transfer flavoprotein + H⁺ (EC 1.3.8.5)

No genes

(2S)-2-methylbutanoyl-CoA + H₂O→2-methylbutanoate + coenzyme A + H⁺

No genes

Links:

Pathway details at MetaCyc
Fitness data for 52 genes
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All MetaCyc pathways for Variovorax sp. OAS795

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information