MetaCyc Pathway: L-isoleucine biosynthesis II in Variovorax sp. OAS795

Add experiment(s):


pyruvate + acetyl-CoA + H2O→(R)-citramalate + coenzyme A + H+ (EC 2.3.3.21)
No genes
(R)-2-methylmalate dehydratase:
(R)-citramalate→citraconate + H2O (EC 4.2.1.35)
ABID97_RS08460
ABID97_RS08465
ABID97_RS27515
ABID97_RS29025
ABID97_RS29030
citraconate + H2O→(2R,3S)-3-methylmalate
ABID97_RS08465
ABID97_RS29025
ABID97_RS29030
(2R,3S)-3-methylmalate + NAD+→2-oxobutanoate + CO2 + NADH
ABID97_RS08455
ABID97_RS29035
Acetolactate synthase:
2-oxobutanoate + pyruvate + H+→(S)-2-aceto-2-hydroxybutanoate + CO2 (EC 2.2.1.6)
ABID97_RS04085
ABID97_RS12545
ABID97_RS13280
ABID97_RS14885
ABID97_RS14890
(S)-2-aceto-2-hydroxybutanoate + NAD(P)H + H+→(R)-2,3-dihydroxy-3-methylpentanoate + NAD(P)+ (EC 1.1.1.383)
No genes
Dihydroxy-acid dehydratase:
(R)-2,3-dihydroxy-3-methylpentanoate→(3S)-3-methyl-2-oxopentanoate + H2O (EC 4.2.1.9)
ABID97_RS03710
ABID97_RS06870
ABID97_RS17500
ABID97_RS21205
ABID97_RS25645
ABID97_RS25870
Branched-chain-amino-acid transaminase (in reverse):
(3S)-3-methyl-2-oxopentanoate + L-glutamate→L-isoleucine + 2-oxoglutarate (EC 2.6.1.42)
ABID97_RS03790

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information