MetaCyc Pathway: gallate degradation III (anaerobic) in Variovorax sp. OAS795

MetaCyc Pathway: gallate degradation III (anaerobic) in Variovorax sp. OAS795

Gallate decarboxylase:
gallate + H⁺→pyrogallol + CO₂ (EC 4.1.1.59)

No genes

Pyrogallol hydroxytransferase:
pyrogallol + 1,2,3,5-tetrahydroxybenzene→phloroglucinol + 1,2,3,5-tetrahydroxybenzene (EC 1.97.1.2)

No genes

Phloroglucinol reductase (in reverse):
phloroglucinol + NADPH + H⁺→dihydrophloroglucinol + NADP⁺ (EC 1.3.1.57)

No genes

dihydrophloroglucinol + H₂O→3-hydroxy-5-oxohexanoate + H⁺

No genes

Acetate CoA-transferase (in reverse):
3-hydroxy-5-oxohexanoate + acetyl-CoA→acetate + 3-hydroxy-5-oxohexanoyl-CoA (EC 2.8.3.8)

No genes

3-hydroxy-5-oxohexanoyl-CoA + coenzyme A→acetyl-CoA + (S)-3-hydroxybutanoyl-CoA

No genes

(S)-3-hydroxybutanoyl-CoA→crotonyl-CoA + H₂O (EC 4.2.1.150)

crotonyl-CoA_[cytosol] + 2 NADH_[cytosol] + 2 an oxidized ferredoxin [iron-sulfur] cluster_[cytosol]→butanoyl-CoA_[cytosol] + 2 NAD⁺_[cytosol] + 2 a reduced ferredoxin [iron-sulfur] cluster_[cytosol] (EC 1.3.1.109)

No genes

Propionate CoA-transferase:
acetate + butanoyl-CoA→acetyl-CoA + butanoate (EC 2.8.3.1; 2.8.3.8)

No genes

Phosphate acetyltransferase:
acetyl-CoA + phosphate→acetyl phosphate + coenzyme A (EC 2.3.1.8)

Acetate kinase (in reverse):
acetyl phosphate + ADP→acetate + ATP (EC 2.7.2.1; 2.7.2.15)

Links:

Pathway details at MetaCyc
Fitness data for 32 genes
Select organism:
All MetaCyc pathways for Variovorax sp. OAS795

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information