MetaCyc Pathway: L-leucine degradation I in Variovorax sp. OAS795

MetaCyc Pathway: L-leucine degradation I in Variovorax sp. OAS795

Branched-chain-amino-acid transaminase:
L-leucine + 2-oxoglutarate→4-methyl-2-oxopentanoate + L-glutamate (EC 2.6.1.42; 2.6.1.6)

2-oxoisovalerate dehydrogenase (acylating):
4-methyl-2-oxopentanoate + coenzyme A + NAD⁺→3-methylbutanoyl-CoA + CO₂ + NADH (EC 1.2.1.25)

No genes

3-methylbutanoyl-CoA + an oxidized electron-transfer flavoprotein + H⁺→3-methylcrotonyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.4)

Methylcrotonoyl-CoA carboxylase:
3-methylcrotonyl-CoA + ATP + hydrogen carbonate→3-methylglutaconyl-CoA + ADP + H⁺ + phosphate (EC 6.4.1.4)

Methylglutaconyl-CoA hydratase (in reverse):
3-methylglutaconyl-CoA + H₂O→(S)-3-hydroxy-3-methylglutaryl-CoA (EC 4.2.1.18)

Hydroxymethylglutaryl-CoA lyase:
(S)-3-hydroxy-3-methylglutaryl-CoA→acetoacetate + acetyl-CoA (EC 4.1.3.4)

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Pathway details at MetaCyc
Fitness data for 16 genes
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All MetaCyc pathways for Variovorax sp. OAS795

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information